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Enhancing Antibody Response against Small Molecular Hapten with Tobacco Mosaic Virus as a Polyvalent Carrier
- Source :
- ChemBioChem. 16:1279-1283
- Publication Year :
- 2015
- Publisher :
- Wiley, 2015.
-
Abstract
- Virus nanoparticles (VNPs) have been applied as carrier proteins for effective vaccine development. In this paper, we report the usage of tobacco mosaic virus (TMV) as a carrier for the display of the small molecule estriol (E3), a weakly immunogenic hapten. A highly efficient copper (I)-catalyzed azide-alkyne cycloaddition reaction (CuAAC) was performed for the conjugation of E3 onto TMV capsid at tyrosine (Tyr) 139, by which the antigen density could be controlled. The immune properties of these constructs were evaluated in mice. We found that a strong and long-term antibody response was elicited by conjugating a high density of small molecular haptens on TMV through an oligo(ethylene glycol) (OEG) linker, likely due to the effective activation of B-cells. This study suggests that TMV can serve as a promising platform to induce strong humoral immune responses and that the optimized conjugation strategy was critical to produce high quality antibodies.
- Subjects :
- Male
Azides
Ethylene Glycol
Immunoconjugates
viruses
Biochemistry
Virus
Cell Line
Mice
Antigen
Tobacco mosaic virus
Animals
Molecular Biology
Drug Carriers
Mice, Inbred BALB C
Cycloaddition Reaction
biology
Estriol
Chemistry
fungi
Organic Chemistry
Molecular biology
Small molecule
Tobacco Mosaic Virus
Capsid
Alkynes
Antibody Formation
Biophysics
biology.protein
Nanoparticles
Molecular Medicine
Capsid Proteins
Immunization
Antibody
Drug carrier
Haptens
Hapten
Subjects
Details
- ISSN :
- 14394227
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- ChemBioChem
- Accession number :
- edsair.doi.dedup.....edeabf9519e5bd572f93b02fe7407e65
- Full Text :
- https://doi.org/10.1002/cbic.201500028