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Enhancing Antibody Response against Small Molecular Hapten with Tobacco Mosaic Virus as a Polyvalent Carrier

Authors :
Qian Wang
Limin Chen
Jittima Amie Luckanagul
Yuan Lin
Xiaolei Zhang
Xia Zhao
Source :
ChemBioChem. 16:1279-1283
Publication Year :
2015
Publisher :
Wiley, 2015.

Abstract

Virus nanoparticles (VNPs) have been applied as carrier proteins for effective vaccine development. In this paper, we report the usage of tobacco mosaic virus (TMV) as a carrier for the display of the small molecule estriol (E3), a weakly immunogenic hapten. A highly efficient copper (I)-catalyzed azide-alkyne cycloaddition reaction (CuAAC) was performed for the conjugation of E3 onto TMV capsid at tyrosine (Tyr) 139, by which the antigen density could be controlled. The immune properties of these constructs were evaluated in mice. We found that a strong and long-term antibody response was elicited by conjugating a high density of small molecular haptens on TMV through an oligo(ethylene glycol) (OEG) linker, likely due to the effective activation of B-cells. This study suggests that TMV can serve as a promising platform to induce strong humoral immune responses and that the optimized conjugation strategy was critical to produce high quality antibodies.

Details

ISSN :
14394227
Volume :
16
Database :
OpenAIRE
Journal :
ChemBioChem
Accession number :
edsair.doi.dedup.....edeabf9519e5bd572f93b02fe7407e65
Full Text :
https://doi.org/10.1002/cbic.201500028