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Huangqin-tang ameliorates dextran sodium sulphate-induced colitis by regulating intestinal epithelial cell homeostasis, inflammation and immune response
- Source :
- Scientific Reports
- Publication Year :
- 2016
- Publisher :
- Nature Publishing Group, 2016.
-
Abstract
- Huangqin-tang (HQT) is a traditional Chinese medicine (TCM) formula widely used for the treatment of inflammatory bowel disease in China. However, the molecular mechanisms by which HQT protects the colon are unclear. We studied the protective effects of HQT and the underlying mechanisms in an experimental mouse model and in vitro. In vivo, dextran sodium sulphate (DSS)-induced acute and chronic colitis were significantly ameliorated by HQT as gauged by phenotypic, histopathologic and inflammatory manifestations of the disease. Mechanistically, DSS-induced nuclear factor-κB (NF-κB) signalling was inhibited by HQT. Moreover, HQT-treated mice demonstrated significant changes in cell apoptosis, expression of apoptosis-associated genes such as caspase-3, bax, bcl-2, and intestinal permeability. HQT also increased occluding and zonula occludens-1 (ZO-1), inhibited cell proliferation (Ki67), and increased regulatory T cells numbers, protein expression of Foxp3 and IL-10 in the colonic tissue. In vitro, HQT down-regulated production of pro-inflammatory cytokines and supressed the NF-κB signalling pathway in lipopolysaccharides-induced RAW 264.7 macrophages. Our study suggests that HQT plays a critical role in regulating intestinal epithelial cell homeostasis, inflammation and immune response in colitis and offers novel therapeutic options in the management of inflammatory bowel disease.
- Subjects :
- 0301 basic medicine
Anti-Inflammatory Agents
Inflammation
Pharmacology
Inflammatory bowel disease
Article
03 medical and health sciences
Mice
0302 clinical medicine
Immune system
Intestinal mucosa
medicine
Animals
Homeostasis
Immunologic Factors
Colitis
Intestinal Mucosa
Multidisciplinary
Intestinal permeability
Chemistry
Histocytochemistry
Dextran Sulfate
FOXP3
Epithelial Cells
medicine.disease
Mice, Inbred C57BL
Disease Models, Animal
030104 developmental biology
RAW 264.7 Cells
Apoptosis
030220 oncology & carcinogenesis
Cytokines
Female
medicine.symptom
Drugs, Chinese Herbal
Signal Transduction
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....edb2bc0cc8da1ffe09a9fc3d1996ef00