The structural basis for specific decoding of AUA by isoleucine tRNA on the ribosome

Decoding of the AUA isoleucine codon in bacteria and archaea requires modification of a C in the anticodon wobble position of the isoleucine tRNA. Here, we report the crystal structure of the archaeal tRNA2Ile, which contains the modification agmatidine in its anticodon, in complex with the AUA codon on the 70S ribosome. The structure illustrates how agmatidine confers codon specificity for AUA over AUG.
Medical Research Council (Great Britain) (grant U105184332)
Agouron Institute
Wellcome Trust (London, England)
Louis-Jeantet Foundation
National Institutes of Health (U.S.) (grant GM17151)
University of Cambridge (Gates Cambridge Scholarship)
Peterhouse (University of Cambridge)
Boehringer Ingelheim Fonds