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Satratoxin H generates reactive oxygen species and lipid peroxides in PC12 cells
- Source :
- Biologicalpharmaceutical bulletin. 31(6)
- Publication Year :
- 2008
-
Abstract
- Satratoxin H, a mycotoxin, is thought to induce apoptosis of PC12 cells through the activation of p38 mitogen-activated protein kinase (MAPK) and c-Jun N-terminal kinase (JNK) in a glutathione (GSH)-sensitive manner. The present study was undertaken to further elucidate the mechanism by which satratoxin H induces cell death in PC12 cells. Satratoxin H caused apoptosis of PC12 cells within 24-h, as determined by DNA fragmentation and flow cytometric analysis. Satratoxin H increased reactive oxygen species (ROS) production and lipid peroxidation, as determined by malondialdehyde formation. These effects were attenuated by incubation of cells with GSH, suggesting that satratoxin H-induced increase in apoptosis of serum-deprived PC12 cells may be partially mediated through the generation of ROS.
- Subjects :
- Programmed cell death
Cell Survival
Pharmaceutical Science
DNA Fragmentation
Biology
PC12 Cells
Thiobarbituric Acid Reactive Substances
p38 Mitogen-Activated Protein Kinases
Antioxidants
Lipid peroxidation
chemistry.chemical_compound
Animals
Protein kinase A
Pharmacology
chemistry.chemical_classification
Electrophoresis, Agar Gel
Reactive oxygen species
Lipid peroxide
fungi
G1 Phase
JNK Mitogen-Activated Protein Kinases
General Medicine
Glutathione
Malondialdehyde
Flow Cytometry
Cell biology
Rats
chemistry
Apoptosis
Indicators and Reagents
Lipid Peroxidation
Reactive Oxygen Species
Trichothecenes
Subjects
Details
- ISSN :
- 09186158
- Volume :
- 31
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Biologicalpharmaceutical bulletin
- Accession number :
- edsair.doi.dedup.....eda7f51fa137e25d23ba585e16d430fa