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A method for cultivation, fractionation, and metal determination to evaluate metal removal by the combination of NPs and bacteria
- Source :
- MethodsX, MethodsX, Vol 8, Iss, Pp 101550-(2021)
- Publication Year :
- 2021
- Publisher :
- Elsevier, 2021.
-
Abstract
- Metals being released into the environment are posing an increasing risk to both environments and public health. Developing improved approaches to remove these metals from the environment is urgent. A current publication discovered that metal bioremediation was significantly improved by nanoparticles (NPs), and the remediation duration was shortened. However, there is no relevant method for the preparation and evaluation of this novel idea. Hence, we developed this method for bacteria in-situ-EPS (Extracellular Polymeric Substances) cultivation, bacteria sub-cellular fractionation, and metal determinations in cultivating solution, EPS and different fractions of bacteria to evaluate metal removal by the combination of NPs and bacteria, including (1) the enhancement of metal bioremediation by NPs, (2) the influence of NPs on bacteria growth and metal toxicity alleviation, (3) the ability of EPS to adsorb metals and the influence of NPs on the EPS metal adsorption, (4) the contribution of bacteria to metal removal in different part, the effects of NPs on metal distribution patterns in bacteria, and the role of NPs in this process.•The design and experimental procedure for the evaluation of metal removal by combing bacteria and NPs.•In-situ EPS cultivation and separation in the study of bioremediation for metals.<br />Graphical abstract Image, graphical abstract
- Subjects :
- inorganic chemicals
Environmental remediation
Science
Subcellular fractionation
Clinical Biochemistry
Metal toxicity
Bacterial growth
Metal
Extracellular polymeric substance
Bioremediation
Adsorption
Nanotechnology
In-situ-EPS
biology
Chemistry
technology, industry, and agriculture
Method Article
biology.organism_classification
Pollution control
Medical Laboratory Technology
Evaluation method for metals removal efficiency by bacteria and nanoparticles
Heavy metals
Environmental chemistry
visual_art
visual_art.visual_art_medium
Bacteria
Subjects
Details
- Language :
- English
- ISSN :
- 22150161
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- MethodsX
- Accession number :
- edsair.doi.dedup.....ed8d343bc5303d0ad6c4513ab9478e2c