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Role of Daptomycin on Burn Wound Healing in an Animal Methicillin-Resistant Staphylococcus aureus Infection Model
- Source :
- Antimicrobial Agents and Chemotherapy. 61
- Publication Year :
- 2017
- Publisher :
- American Society for Microbiology, 2017.
-
Abstract
- Prolonged hospitalization and antibiotic therapy are risk factors for the development of methicillin-resistant Staphylococcus aureus (MRSA) infections in thermal burn patients. We used a rat model to study the in vivo efficacy of daptomycin in the treatment of burn wound infections by S. aureus , and we evaluated the wound healing process through morphological and immunohistochemical analysis. A copper bar heated in boiling water was applied on a paraspinal site of each rat, resulting in two full-thickness burns. A small gauze was placed over each burn and inoculated with 5 × 10 7 CFU of S. aureus ATCC 43300. The study included two uninfected control groups with and without daptomycin treatment, an infected control group that did not receive any treatment, and two infected groups treated, respectively, with intraperitoneal daptomycin and teicoplanin. The main outcome measures were quantitative culture, histological evaluation of tissue repair, and immunohistochemical expression of wound healing markers: epidermal growth factor receptor (EGFR) and fibroblast growth factor 2 (FGF-2). The highest inhibition of infection was achieved in the group that received daptomycin, which reduced the bacterial load from 10 7 CFU/ml to about 10 3 CFU/g ( P < 0.01). The groups treated with daptomycin showed better overall healing with epithelialization and significantly higher collagen scores than the other groups, and these findings were also confirmed by immunohistochemical data. In conclusion, our results support the hypothesis that daptomycin is an important modulator of wound repair by possibly reducing hypertrophic burn scar formation.
- Subjects :
- Male
Methicillin-Resistant Staphylococcus aureus
0301 basic medicine
medicine.medical_specialty
030106 microbiology
Microbial Sensitivity Tests
Fibroblast growth factor
medicine.disease_cause
Gastroenterology
Cicatrix
03 medical and health sciences
Daptomycin
In vivo
Internal medicine
Animals
Medicine
Pharmacology (medical)
Rats, Wistar
Mechanisms of Action: Physiological Effects
Cell Proliferation
Pharmacology
Wound Healing
business.industry
Teicoplanin
Epithelial Cells
Staphylococcal Infections
Methicillin-resistant Staphylococcus aureus
Bacterial Load
Thermal burn
Anti-Bacterial Agents
Rats
ErbB Receptors
Disease Models, Animal
030104 developmental biology
Infectious Diseases
Staphylococcus aureus
Wound Infection
Fibroblast Growth Factor 2
Burns
Wound healing
business
medicine.drug
Subjects
Details
- ISSN :
- 10986596 and 00664804
- Volume :
- 61
- Database :
- OpenAIRE
- Journal :
- Antimicrobial Agents and Chemotherapy
- Accession number :
- edsair.doi.dedup.....ed84e025e81ab31e11cc998e730945a6