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Distribution of type VI collagen in association with osteoblast lineages in the groove of Ranvier during rat postnatal development
- Source :
- Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft. 208
- Publication Year :
- 2015
-
Abstract
- In the groove of Ranvier (GOR), osteoblast lineages form bone bark, which develops into endosteal cortical bone. This ossification process is thought to be regulated by the microenvironment in the GOR. Type VI collagen (Col VI), an extracellular matrix (ECM) protein found in the periosteum/perichondrium, mediates osteoblast differentiation via the cell-surface receptor neural/glial antigen 2 (NG2) chondroitin sulfate proteoglycan. In order to clarify the function of Col VI during osteoblast differentiation in the GOR, in the present study, we examined the distribution of Col VI and osteoblast lineages expressing NG2 in the rat tibia proximal end during postnatal growing periods by immunohistochemistry. Our data revealed that Col VI accumulated in the ECM of the GOR middle layer and that Col VI accumulation was reduced and disappeared in the inner and middle lower regions. Runt-related transcription factor 2-immunoreactive pre-osteoblasts expressed NG2 in Col VI-immunopositive areas. However, Osterix-immunoreactive mature osteoblasts were only found in the Col VI-immunonegative area. These findings indicate that Col VI provided a characteristic microenvironment in the GOR and that NG2-Col VI interactions may regulate the differentiation of osteoblast lineages prior to terminal maturation.
- Subjects :
- musculoskeletal diseases
0301 basic medicine
Male
Aging
SOX9
Collagen Type VI
Extracellular matrix
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
medicine
Perichondrium
Animals
Cell Lineage
Tissue Distribution
Growth Plate
Antigens
Rats, Wistar
Receptor
Osteoblasts
Chemistry
Ossification
Gene Expression Regulation, Developmental
Osteoblast
Cell Differentiation
General Medicine
Cell biology
Rats
RUNX2
030104 developmental biology
medicine.anatomical_structure
nervous system
Chondroitin sulfate proteoglycan
Immunology
Proteoglycans
Anatomy
medicine.symptom
030217 neurology & neurosurgery
Developmental Biology
Subjects
Details
- ISSN :
- 16180402
- Volume :
- 208
- Database :
- OpenAIRE
- Journal :
- Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft
- Accession number :
- edsair.doi.dedup.....ed7c2ee22d25d9a3f8f5bbd4dc3c5006