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Gene-Based Sequence Diversity Analysis of Field Pea (Pisum)
- Publication Year :
- 2007
- Publisher :
- Genetics Society of America, 2007.
-
Abstract
- Sequence diversity of 39 dispersed gene loci was analyzed in 48 diverse individuals representative of the genus Pisum. The different genes show large variation in diversity parameters, suggesting widely differing levels of selection and a high overall diversity level for the species. The data set yields a genetic diversity tree whose deep branches, involving wild samples, are preserved in a tree derived from a polymorphic retrotransposon insertions in an identical sample set. Thus, gene regions and intergenic “junk DNA” share a consistent picture for the genomic diversity of Pisum, despite low linkage disequilibrium in wild and landrace germplasm, which might be expected to allow independent evolution of these very different DNA classes. Additional lines of evidence indicate that recombination has shuffled gene haplotypes efficiently within Pisum, despite its high level of inbreeding and widespread geographic distribution. Trees derived from individual gene loci show marked differences from each other, and genetic distance values between sample pairs show high standard deviations. Sequence mosaic analysis of aligned sequences identifies nine loci showing evidence for intragenic recombination. Lastly, phylogenetic network analysis confirms the non-treelike structure of Pisum diversity and indicates the major germplasm classes involved. Overall, these data emphasize the artificiality of simple tree structures for representing genomic sequence variation within Pisum and emphasize the need for fine structure haplotype analysis to accurately define the genetic structure of the species.
- Subjects :
- Genetics
Recombination, Genetic
Genetic diversity
Linkage disequilibrium
Base Sequence
Retroelements
Molecular Sequence Data
Peas
food and beverages
Genetic Variation
Phylogenetic network
Biology
Investigations
Genes, Plant
Linkage Disequilibrium
Intergenic region
Genetic distance
Genetic variation
Genetic structure
Hordeum vulgare
Selection, Genetic
human activities
Phylogeny
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....ed7339758b27f845c5a54c7f55309f3f