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Interleukin-15 favors the expansion of central memory CD8+ T cells in ex vivo generated, antileukemia human cytotoxic T lymphocyte lines
- Source :
- Journal of immunotherapy (Hagerstown, Md. : 1997). 31(4)
- Publication Year :
- 2008
-
Abstract
- We demonstrated in previous studies that interleukin (IL) -2 supports in vitro cell proliferation of donor-derived cytotoxic T lymphocyte (CTL) lines directed against different types of leukemia blasts. The aim of this study was to compare the capacity of IL-15 with that of IL-2 in supporting the proliferation and cytotoxic activity of antileukemia CTL cultures, and their influence on T-cell memory compartment differentiation. Antileukemia CTL lines were generated using donor-derived dendritic cells pulsed with apoptotic leukemia blasts, in the presence of IL-12 and IL-7, during the primary culture, and expanded through 2 rounds of leukemia-specific stimulation and 1 round of antigen-independent expansion, each supplemented with either IL-2 or IL-15. Both IL-2-supplemented (IL-2-CTLs) and IL-15-supplemented (IL-15-CTLs) lines contained predominant numbers of CD45RA/CCR7 effector memory (TEM) and CD45RA/CCR7 (TEMRA+) T cells. Significantly higher numbers (P
- Subjects :
- Cytotoxicity, Immunologic
Male
Cancer Research
Adolescent
Immunology
chemical and pharmacologic phenomena
Biology
CD8-Positive T-Lymphocytes
Lymphocyte Activation
Immunotherapy, Adoptive
Immunophenotyping
Interleukin 21
Interferon-gamma
Antigen
medicine
Immunology and Allergy
Cytotoxic T cell
Humans
Interferon gamma
Child
Cells, Cultured
Pharmacology
Interleukin-15
Interleukin
hemic and immune systems
T lymphocyte
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Molecular biology
CTL
Leukemia, Myeloid, Acute
Interleukin 15
Child, Preschool
Female
Immunologic Memory
medicine.drug
Subjects
Details
- ISSN :
- 15249557
- Volume :
- 31
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Journal of immunotherapy (Hagerstown, Md. : 1997)
- Accession number :
- edsair.doi.dedup.....ed57f58f61fd499083362b0e225c2003