Contrasting strategies to cope with chilling stress among clones of a tropical tree, Hevea brasiliensis

Eight Hevea brasiliensis Muell. Arg. clones (GT1, YUNYAN77-4, IRCA707, IRCA317, PB217, PB260, PR107 and RRIM600) were compared for their tolerance towards chilling stress. Net photosynthesis (Pn), stomatal conductance (Gs), optimal and effective photochemical efficiencies (F(v)/F(m) and F'(v) = F'(m)), non-photochemical quenching, cellular lysis and leaf necrosis were measured on trees chilled at 10 degrees C for 96 h, as well as upon recovery at 28 degrees C. In addition, ascorbate peroxidase, catalase, dehydroascorbate reductase, glutathione reductase, monodehydroascorbate reductase and superoxide dismutase activities were monitored. Clone RRIM600 appeared to be the most tolerant, because it showed no cellular lysis or leaf necrosis and the best recovery as revealed by Pn, Gs, Fv/Fm and F'(v) = F'(m). Its ability to sustain chilling stress seemed related in part to the fast closure of stomata, suggesting an 'avoidance strategy' for this clone. IRCA707, GT1 and YUNYAN77-4 were also tolerant to the cold treatment as only a few leaf injuries were observed. However, YUNYAN77-4 showed a particular behaviour with a large stomata opening during the first hour of chilling, some photosynthetic activity after 96 h at 10 degrees C, but the slowest recovery in Pn. The greatest cell or leaf damage was observed on PB260, IRCA317, PR107 and PB217 clones, thus classified as sensitive to chilling. These clones showed the strongest decrease in Pn, F(v)/F(m) and F'(v) = F'(m) and the slowest recovery for F(v)/F(m) and F'(v) = F'(m), indicating a high sensitivity of photosystem II to cold temperatures. Punctual increases of various enzymatic activities were observed for all clones during chilling kinetics. During recovery, the strongest increases in enzymatic activity were observed for the most tolerant clones, suggesting that efficient reactive oxygen species elimination is a crucial step for determining chilling tolerance in Hevea although the enzymes implicated varied from one tolerant clone to another. This study points out contrasted strategies of the Hevea clones in copping with chilling stress and recovery.