Back to Search
Start Over
Production and characterization of engineered alginate-based microparticles containing ECM powder for cell/tissue engineering applications
- Source :
- Università degli studi di Ferrara-IRIS
-
Abstract
- A method for the production of engineered alginate-based microparticles, containing extracellular matrix and neonatal porcine Sertoli cells (SCs), is described. As a source for extracellular matrix, a powder form of isolated and purified urinary bladder matrix (UBM) was employed. We demonstrated that the incorporation of UBM does not significantly alter the morphological and dimensional characteristics of the microparticles. The alginate microparticles were used for SC encapsulation as an immunoprotective barrier for transplant purposes, while the co-entrapped UBM promoted retention of cell viability and function. These engineered microparticles could represent a novel approach to enhancing immunological acceptance and increasing the functional life-span of the entrapped cells for cell/tissue engineering applications. In this respect, it is noteworthy that isolated neonatal porcine SCs, administered alone in highly biocompatible microparticles, led to diabetes prevention and reversion in nonobese diabetic (NOD) mice.
- Subjects :
- Male
Materials science
Alginates
Swine
Cell
Urinary Bladder
Biomedical Engineering
Nod
Microparticles
Biochemistry
Biomaterials
Extracellular matrix
Tissue engineering
Glucuronic Acid
medicine
Animals
Viability assay
Urinary bladder matrix
Cell encapsulation
Molecular Biology
SCs
DNA Primers
Base Sequence
Tissue Engineering
Alginate
Reverse Transcriptase Polymerase Chain Reaction
Hexuronic Acids
General Medicine
Biocompatible material
Sertoli cell
Microspheres
Cell biology
Extracellular Matrix
medicine.anatomical_structure
Powders
Biotechnology
Biomedical engineering
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Università degli studi di Ferrara-IRIS
- Accession number :
- edsair.doi.dedup.....ed3bf434e9b67d05908be64aafa1807d