Back to Search
Start Over
HIV-1 DNA Levels in Peripheral Blood Mononuclear Cells and Cannabis Use are Associated With Intermittent HIV Shedding in Semen of Men Who Have Sex With Men on Successful Antiretroviral Regimens
- Source :
- Clinical Infectious Diseases. 58:1763-1770
- Publication Year :
- 2014
- Publisher :
- Oxford University Press (OUP), 2014.
-
Abstract
- Few data exist on the efficacy of combined antiretroviral therapy (cART) in semen of human immunodeficiency virus type 1 (HIV-1) infected men who have sex with men (MSM) with sustained control of HIV replication in blood.HIV-1 infected MSM on successful cART for6 months were enrolled. HIV-RNA was quantified in seminal plasma (spVL) and in blood plasma (bpVL) from 2 paired samples collected 4 weeks apart. Relationship between spVL and bpVL (measured by an ultrasensitive assay, LOQ 10 copies/mL), total peripheral blood mononuclear cells (PBMC)-associated HIV-DNA, sexually transmitted infections (STIs), and self-reported socio-behavioral characteristics was assessed using GEE logistic regression.In total, 157 patients were included. Median time with bpVL50 copies/mL was 3.3 years. spVL was detectable in 23/304 samples (prevalence 7.6%). Median spVL was 145 cp/mL (100-1475). spVL was detectable on the first, on the second, and on both samples in 5, 14, and 2 men, respectively. In sum, 33 individuals (21%) had STIs (asymptomatic in 24/33). Residual bpVL was undetectable by ultrasensitive assay in 225/300 samples (75%). After multivariable adjustments, PBMC-associated HIV-DNA (OR 2.6[1.2; 6.0], for HIV-DNA2.5 log10 cp/10(6) PBMC, P = .02), and cannabis use during sexual intercourse (OR 2.8[1.2; 6.7], P = .02) were the only factors associated significantly with spVL.We show that HIV-RNA can be detected intermittently in semen of HIV-1 infected MSM despite successful cART. The size of blood HIV-1 reservoir predicted spVL detection. Our results indicated also that the possible effect of cannabis should be taken into account when developing prevention interventions targeted toward HIV-infected MSM on successful cART.
- Subjects :
- Adult
Male
Sexually Transmitted Diseases, Bacterial
Microbiology (medical)
Cart
medicine.medical_specialty
Sexual transmission
Anti-HIV Agents
HIV Infections
Marijuana Smoking
Semen
Men who have sex with men
Acquired immunodeficiency syndrome (AIDS)
Internal medicine
Blood plasma
medicine
Humans
Homosexuality, Male
Sida
biology
business.industry
virus diseases
Middle Aged
Viral Load
biology.organism_classification
medicine.disease
Virus Shedding
Infectious Diseases
DNA, Viral
Immunology
HIV-1
Leukocytes, Mononuclear
RNA, Viral
Drug Therapy, Combination
Viral disease
business
Subjects
Details
- ISSN :
- 15376591 and 10584838
- Volume :
- 58
- Database :
- OpenAIRE
- Journal :
- Clinical Infectious Diseases
- Accession number :
- edsair.doi.dedup.....ed07677e89594ffe68340097837ff3b7
- Full Text :
- https://doi.org/10.1093/cid/ciu187