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Reductively Degradable Poly(2-hydroxyethyl methacrylate) Hydrogels with Oriented Porosity for Tissue Engineering Applications
- Source :
- ACS applied materialsinterfaces. 9(12)
- Publication Year :
- 2017
-
Abstract
- Degradable poly(2-hydroxyethyl methacrylate) hydrogels were prepared from a linear copolymer (Mw = 49 kDa) of 2-hydroxyethyl methacrylate (HEMA), 2-(acethylthio)ethyl methacrylate (ATEMA), and zwitterionic 2-methacryloyloxyethyl phosphorylcholine (MPC). The deprotection of ATEMA thiol groups by triethylamine followed by their gentle oxidation with 2,2'-dithiodipyridine resulted in the formation of reductively degradable polymers with disulfide bridges. Finally, a hydrogel 3D structure with an oriented porosity was obtained by gelation of the polymer in the presence of needle-like sodium acetate crystals. The pore diameter and porosity of resulting poly(2-hydroxyethyl methacrylate-co-2-(acethylthio)ethyl methacrylate-co-2-methacryloyloxyethyl phosphorylcholine) [P(HEMA-ATEMA-MPC)] hydrogels varied between 59 and 65 μm and between 70 and 79.6 vol % according to Hg porosimetry, and complete degradation of these materials was reached in 86 days in 0.33 mmol solution of l-cysteine/L in phosphate buffer. The cross-linked P(HEMA-ATEMA-MPC) hydrogels were evaluated as a possible support for human mesenchymal stem cells (MSCs). No cytotoxicity was found for the un-cross-linked thiol-containing and protected P(HEMA-ATEMA-MPC) chains up to a concentration of 5 and 1 wt % in α-minimum essential medium, respectively.
- Subjects :
- chemistry.chemical_classification
Materials science
Tissue Engineering
Hydrogels
02 engineering and technology
Polymer
010402 general chemistry
021001 nanoscience & nanotechnology
Methacrylate
01 natural sciences
0104 chemical sciences
2-Hydroxyethyl Methacrylate
chemistry.chemical_compound
chemistry
Polymer chemistry
Self-healing hydrogels
Thiol
Copolymer
Humans
General Materials Science
0210 nano-technology
Triethylamine
Sodium acetate
Porosity
Polyhydroxyethyl Methacrylate
Subjects
Details
- ISSN :
- 19448252
- Volume :
- 9
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- ACS applied materialsinterfaces
- Accession number :
- edsair.doi.dedup.....ed00b4aa05a3d8fec07510f99a2e0457