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Anti-Müllerian hormone production in the ovary: a comparative study in bovine and porcine granulosa cells†
- Source :
- Biology of Reproduction, Biology of Reproduction, Society for the Study of Reproduction, 2020, 103 (3), pp.572-582. ⟨10.1093/biolre/ioaa077⟩
- Publication Year :
- 2020
- Publisher :
- Oxford University Press (OUP), 2020.
-
Abstract
- In this study, we aimed to determine the origin of the difference, in terms of anti-Müllerian hormone production, existing between the bovine and porcine ovaries. We first confirmed by quantitative real-time-Polymerase-Chain Reaction, ELISA assay and immunohistochemistry that anti-Müllerian hormone mRNA and protein production are very low in porcine ovarian growing follicles compared to bovine ones. We then have transfected porcine and bovine granulosa cells with vectors containing the luciferase gene driven by the porcine or the bovine anti-Müllerian hormone promoter. These transfection experiments showed that the porcine anti-Müllerian hormone promoter is less active and less responsive to bone morphogenetic protein stimulations than the bovine promoter in both porcine and bovine cells. Moreover, bovine but not porcine granulosa cells were responsive to bone morphogenetic protein stimulation after transfection of a plasmidic construction including a strong response element to the bone morphogenetic proteins (12 repetitions of the GCCG sequence) upstream of the luciferase reporter gene. We also showed that SMAD6, an inhibitor of the SMAD1-5-8 pathway, is strongly expressed in porcine compared to the bovine granulosa cells. Overall, these results suggest that the low expression of anti-Müllerian hormone in porcine growing follicles is due to both a lack of activity/sensitivity of the porcine anti-Müllerian hormone promoter, and to the lack of responsiveness of porcine granulosa cells to bone morphogenetic protein signaling, potentially due to an overexpression of SMAD6 compared to bovine granulosa cells. We propose that the low levels of anti-Müllerian hormone in the pig would explain the poly-ovulatory phenotype in this species.
- Subjects :
- Anti-Mullerian Hormone
pig
Smad6 Protein
Swine
[SDV]Life Sciences [q-bio]
Response element
Ovary
Biology
Bone morphogenetic protein
03 medical and health sciences
granulosa
0302 clinical medicine
Species Specificity
AMH
Protein biosynthesis
medicine
Animals
Promoter Regions, Genetic
030304 developmental biology
0303 health sciences
Messenger RNA
Granulosa Cells
promoter
030219 obstetrics & reproductive medicine
Gene Expression Regulation, Developmental
[SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology
Anti-Müllerian hormone
Cell Biology
General Medicine
Transfection
Immunohistochemistry
Molecular biology
medicine.anatomical_structure
Reproductive Medicine
cattle
Bone Morphogenetic Proteins
biology.protein
Female
Signal Transduction
Hormone
Subjects
Details
- ISSN :
- 15297268 and 00063363
- Volume :
- 103
- Database :
- OpenAIRE
- Journal :
- Biology of Reproduction
- Accession number :
- edsair.doi.dedup.....ecf6171e11d9a1cf81f4c8c5733c55ab