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Sex differentiation and mRNA expression of P450c17, P450arom and AMH in gonads of the chicken
- Source :
- Molecular reproduction and development. 55(1)
- Publication Year :
- 1999
-
Abstract
- The present study was conducted to reveal effects of in ovo injection of nonsteroidal aromatase inhibitor (Fadrozole) or estradiol at day 3 of incubation on mRNA levels of P45017ahydroxylase (P450c17), P450 aromatase (P450arom) and anti- Mullerian hormone (AMH) in the chicken gonads. The mRNA levels in the gonads at days 4-8 of incubation were assessed by in situ hybridization analysis using digoxigenin labeling method. The in situ hybridization data were analyzed by relative expression of specific hybridizable signals of each mRNA corrected by the non-specific background by employing an image ana- lyzer. P450c17 mRNA expression increased rapidly at day 6 of incubation in the male but decreased thereaf- ter. In contrast to the transient expression in the male, the expression was gradually increased in the female. P450arom mRNA was not expressed in the male but was detectable in the female as early as day 6 and increased subsequently with days of incubation. AMH mRNA was expressed as early as day 5 of incubation followed by a sharp increase on day 6, which was maintained in the male thereafter. In contrast, the female showed very little expression. The injection of Fadrozole caused no effect on P450c17 mRNA expres- sion, while it suppressed P450arom mRNA expression but increased AMH mRNA expression in the female. In contrast, the injection of estradiol induced P450arom mRNA expression significantly but suppressed AMH mRNA expression in the male. These results indicate that expression of P450arom and AMH is sexually dimorphic and is reciprocally regulated during early ontogenic life in chicken gonads. Mol. Reprod. Dev. 55:20-30, 2000. r 2000 Wiley-Liss, Inc.
- Subjects :
- Anti-Mullerian Hormone
Male
endocrine system
medicine.medical_specialty
Gonad
Sex Differentiation
Time Factors
medicine.drug_class
Gene Expression
In situ hybridization
Chick Embryo
Biology
In ovo
Aromatase
Sex Factors
Internal medicine
Gene expression
Genetics
medicine
Animals
Gonads
Incubation
In Situ Hybridization
Glycoproteins
Sexual differentiation
Aromatase Inhibitors
Steroid 17-alpha-Hydroxylase
Cell Biology
Blotting, Northern
Growth Inhibitors
Testicular Hormones
Fadrozole
Endocrinology
medicine.anatomical_structure
Estrogen
Female
Developmental Biology
medicine.drug
Subjects
Details
- ISSN :
- 1040452X
- Volume :
- 55
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Molecular reproduction and development
- Accession number :
- edsair.doi.dedup.....ecf08ee66262038f1d95a29e4d9b66bb