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Suppression effect of LHRH-A and hCG on Piwi expression in testis of Nile tilapia Oreochromis niloticus
- Source :
- General and Comparative Endocrinology. 189:43-50
- Publication Year :
- 2013
- Publisher :
- Elsevier BV, 2013.
-
Abstract
- Piwi proteins as constituent factors of the piRNA pathway are required for germline maintenance, meiosis and gonad development. Previous study showed hCG could regulate the Piwi expression in ovary of teleosts. In this study, we revealed effects of LHRH-A and hCG on Piwi expression in testis of tilapia using Real-time PCR and Western blot. Both in vivo and in vitro study suggest that LHRH-A and hCG significantly down-regulated Piwil-1 and Piwil-2 in mRNA or protein levels compared with controls. Meanwhile, tissue and cell distribution showed that Piwi proteins were mainly expressed in spermatocytes rather than mature sperms. These results indicated that HPG suppresses Piwis which may play a crucial role in testis differentiation and development.
- Subjects :
- Male
endocrine system
Piwi-interacting RNA
Ovary
Biology
Chorionic Gonadotropin
Germline
Gonadotropin-Releasing Hormone
Nile tilapia
Endocrinology
Western blot
Testis
medicine
Animals
Humans
Regulation of gene expression
Messenger RNA
medicine.diagnostic_test
urogenital system
Gene Expression Regulation, Developmental
Cichlids
biology.organism_classification
Molecular biology
Cell biology
medicine.anatomical_structure
Argonaute Proteins
Animal Science and Zoology
Development of the gonads
hormones, hormone substitutes, and hormone antagonists
Subjects
Details
- ISSN :
- 00166480
- Volume :
- 189
- Database :
- OpenAIRE
- Journal :
- General and Comparative Endocrinology
- Accession number :
- edsair.doi.dedup.....ecad49bf4685875647ea56279d362fd5