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Circular RNA circ-RanGAP1 regulates VEGFA expression by targeting miR-877-3p to facilitate gastric cancer invasion and metastasis
- Source :
- Cancer letters. 471
- Publication Year :
- 2019
-
Abstract
- The biological functions of circular RNAs (circRNAs) in gastric cancer (GC) remain largely unexplored. Here, we identified that circ-RanGAP1 was significantly upregulated in both GC tissues and exosomes from the plasma of GC patients. High circ-RanGAP1 expression was closely associated with an advanced TNM stage, lymph node metastases, and worse survival. Inhibition of circ-RanGAP1 decreased GC cell invasion and migration in vitro. Overexpression of circ-RanGAP1 had the opposite effect. Additionally, circ-RanGAP1 silencing remarkably suppressed tumor growth and metastasis of GC in vivo. Mechanistically, circ-RanGAP1 sponged miR-877-3p to upregulate VEGFA expression. Overexpression of miR-877-3p reversed the biological functions mediated by circ-RanGAP1 in GC cells. Interestingly, we demonstrated that circ-RanGAP1 was upregulated in plasma exosomes from preoperative GC patients. More importantly, the plasma exosomes derived from these patients enhanced the migration and invasion potential of GC cells. Overall, the circ-RanGAP1-mediated miR-877-3p/VEGFA axis promotes GC progression. Our findings suggest that circ-RanGAP1 might act as a potential prognostic biomarker and therapeutic target for GC treatment.
- Subjects :
- 0301 basic medicine
Male
Vascular Endothelial Growth Factor A
Cancer Research
Mice, Nude
Exosomes
Metastasis
03 medical and health sciences
Mice
0302 clinical medicine
Downregulation and upregulation
Stomach Neoplasms
Cell Line, Tumor
medicine
Gene silencing
Animals
Humans
Neoplasm Invasiveness
Neoplasm Metastasis
Lymph node
Aged
Mice, Inbred BALB C
Competing endogenous RNA
Chemistry
GTPase-Activating Proteins
Cancer
RNA, Circular
Middle Aged
medicine.disease
Microvesicles
Vascular endothelial growth factor A
MicroRNAs
030104 developmental biology
medicine.anatomical_structure
Oncology
030220 oncology & carcinogenesis
Cancer research
Heterografts
Female
Subjects
Details
- ISSN :
- 18727980
- Volume :
- 471
- Database :
- OpenAIRE
- Journal :
- Cancer letters
- Accession number :
- edsair.doi.dedup.....eca349758e474d5c7afb6d7d862a85b1