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Dimethylsulfoxide exposure modulates HL-60 cell rolling interactions
- Source :
- Bioscience Reports, Vol 32, Iss 4 (2012), Bioscience Reports
- Publication Year :
- 2012
- Publisher :
- Portland Press, Biochemical Society, 2012.
-
Abstract
- Human leukaemic HL-60 cells are widely used for studying interactions involving adhesion molecules [e.g. P-selectin and PSGL-1 (P-selectin glycoprotein ligand-1)] since their rolling behaviour has been shown to mimic the dynamics of leucocyte rolling in vitro. HL-60 cells are neutrophilic promyelocytes that can undergo granulocytic differentiation upon exposure to compounds such as DMSO (dimethylsulfoxide). Using a parallel plate flow chamber functionalized with recombinant P-selectin–Fc chimaera, undifferentiated and DMSO-induced (48, 72 and 96 h) HL-60 cells were assayed for rolling behaviour. We found that depending on P-selectin incubation concentration, undifferentiated cells incurred up to a 6-fold increase in rolling velocity while subjected to an approximately 10-fold increase in biologically relevant shear stress. HL-60 cells exposed to DMSO for up to 72 h incurred up to a 3-fold increase in rolling velocity over the same shear stress range. Significantly, cells exposed for up to 96 h incurred up to a 9-fold decrease in rolling velocity, compared with undifferentiated HL-60 cells. Although cell surface and nuclear morphological changes were evident upon exposure to DMSO, flow cytometric analysis revealed that PSGL-1 expression was unchanged, irrespective of treatment duration. The results suggest that DMSO-treated HL-60 cells may be problematic as a substitute for neutrophils for trafficking studies during advanced stages of the LAC (leucocyte adhesion cascade). We suggest that remodelling of the cell surface during differentiation may affect rolling behaviour and that DMSO-treated HL-60 cells would behave differently from the normal leucocytes during inflammatory response in vivo.
- Subjects :
- Cytoplasm
leucocyte adhesion cascade (LAC)
LAC, leucocyte adhesion cascade
HL-60 cell rolling interaction
Cell
lcsh:Life
lcsh:QR1-502
ICAM, intercellular adhesion molecule
Biochemistry
PSGL-1, P-selectin glycoprotein ligand-1
FOV, field of view
lcsh:Microbiology
LFA-1, lymphocyte function-associated antigen-1
PE, phycoethryin
DMSO
Membrane Glycoproteins
Cell adhesion molecule
BN, banded nuclei
CD11b, cluster of differentiation molecule 11b
PBA, PBS containing 1% FBS and 0.02% sodium azide
P-Selectin
medicine.anatomical_structure
DMF, dimethylformamide
Parallel-plate flow chamber
Recombinant Fusion Proteins
Biophysics
Mac-1, macrophage-1 antigen
Leukocyte Rolling
HL-60 Cells
Biology
S2
FBS, fetal bovine serum
In vivo
medicine
Cell Adhesion
Humans
Dimethyl Sulfoxide
Cell adhesion
Molecular Biology
Cell Shape
Cell Nucleus
Original Paper
Cell Biology
In vitro
Immunoglobulin Fc Fragments
NT, no treatment
parallel plate flow chamber
lcsh:QH501-531
Immobilized Proteins
GN, cytoplasmic granules
Subjects
Details
- Language :
- English
- ISSN :
- 15734935 and 01448463
- Volume :
- 32
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Bioscience Reports
- Accession number :
- edsair.doi.dedup.....ec936d5105bbc92b82a14ceb96762835