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Emotional arousal state influences the ability of amygdalar endocannabinoid signaling to modulate anxiety
- Publication Year :
- 2016
- Publisher :
- Elsevier Ltd, 2016.
-
Abstract
- Systemic activation of cannabinoid receptors often induces biphasic effects on emotional memory and anxiety depending on the levels of emotional arousal associated to the experimental context. The basolateral nucleus of the amygdala (BLA) represents a crucial structure for the ability of endocannabinoid (eCB) signaling to modulate emotional behaviour, and receives dense projections from brainstem arousal system nuclei. We examined whether changes in emotional arousal state would influence the ability of acute eCB manipulations within the BLA to modulate anxiety. Rats were tested in an elevated plus maze (EPM) under low or high arousal conditions. The low emotional arousal group was extensively handled and habituated to the experimental room and tested under red light condition, the high emotional arousal group was not handled or habituated and tested under high light condition. We examined amygdalar eCB anandamide (AEA) and 2-arachidonoylglycerol (2-AG) levels immediately after the EPM and the effects of intra-BLA administration of the AEA hydrolysis inhibitor URB597 or the 2-AG hydrolysis inhibitor KML29 on anxiety behaviour. The modulation of anxiety-like behaviour by eCBs in the BLA was strictly dependent on the environmental-associated emotional arousal. Pharmacologically-induced elevations of AEA or 2-AG in the BLA decreased anxiety under conditions of low emotional arousal. Conversely, when the level of emotional arousal increased, local eCB manipulation was ineffective in the modulation of the emotional arousal-induced anxiety response. These findings suggest that, depending on the emotional arousal state, eCB system is differentially activated to regulate the anxiety response in the amygdala and help to understand the state-dependency of many interventions on anxiety.
- Subjects :
- Male
0301 basic medicine
Elevated plus maze
Epinephrine
Polyunsaturated Alkamides
Emotions
Context (language use)
Arachidonic Acids
Amygdala
cellular and molecular neuroscience
Glycerides
Developmental psychology
Arousal
emotional arousal
Rats, Sprague-Dawley
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Receptor, Cannabinoid, CB1
medicine
Animals
FAAH
Cannabinoid Receptor Agonists
anxiety
basolateral amygdala
corticosterone
endocannabinoid
pharmacology
Basolateral Nuclear Complex
URB597
Endocannabinoid system
Rats
030104 developmental biology
medicine.anatomical_structure
chemistry
Benzamides
Anxiety
Carbamates
medicine.symptom
Psychology
Neuroscience
030217 neurology & neurosurgery
Endocannabinoids
Signal Transduction
Basolateral amygdala
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....ec872f025d1ee825b273c1027c75f5e4