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Voltage-gated calcium and sodium channels mediate Sema3A retrograde signaling that regulates dendritic development
- Source :
- Brain Research. 1631:127-136
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- Growing axons rely on local signaling at the growth cone for guidance cues. Semaphorin3A (Sema3A), a secreted repulsive axon guidance molecule, regulates synapse maturation and dendritic branching. We previously showed that local Sema3A signaling in the growth cones elicits retrograde retrograde signaling via PlexinA4 (PlexA4), one component of the Sema3A receptor, thereby regulating dendritic localization of AMPA receptor GluA2 and proper dendritic development. In present study, we found that nimodipine (voltage-gated L-type Ca(2+) channel blocker) and tetrodotoxin (TTX; voltage-gated Na(+) channel blocker) suppress Sema3A-induced dendritic localization of GluA2 and dendritic branch formation in cultured hippocampal neurons. The local application of nimodipine or TTX to distal axons suppresses retrograde transport of Venus-Sema3A that has been exogenously applied to the distal axons. Sema3A facilitates axonal transport of PlexA4, which is also suppressed in neurons treated with either TTX or nimodipine. These data suggest that voltage-gated calcium and sodium channels mediate Sema3A retrograde signaling that regulates dendritic GluA2 localization and branch formation.
- Subjects :
- Male
0301 basic medicine
Neurogenesis
Growth Cones
Tetrodotoxin
Biology
Dendritic branch
Axonal Transport
Hippocampus
Sodium Channels
03 medical and health sciences
Animals
Receptors, AMPA
Rats, Wistar
Molecular Biology
Cells, Cultured
Neurons
Voltage-dependent calcium channel
Voltage-gated ion channel
General Neuroscience
Semaphorin-3A
SEMA3A
Dendrites
Rats
Cell biology
030104 developmental biology
nervous system
Axoplasmic transport
Retrograde signaling
Calcium
Female
Nimodipine
Axon guidance
Calcium Channels
Neurology (clinical)
Neuroscience
Synapse maturation
Signal Transduction
Developmental Biology
Subjects
Details
- ISSN :
- 00068993
- Volume :
- 1631
- Database :
- OpenAIRE
- Journal :
- Brain Research
- Accession number :
- edsair.doi.dedup.....ec804b5ae576d6257413168b1f0a327e
- Full Text :
- https://doi.org/10.1016/j.brainres.2015.11.034