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Molecular Dissection of Cucumis metuliferus Resistance against Papaya Ringspot Virus by Grafting
- Source :
- Plants, Vol 9, Iss 1666, p 1666 (2020), Plants, Volume 9, Issue 12
- Publication Year :
- 2020
- Publisher :
- MDPI AG, 2020.
-
Abstract
- Vegetable crops of the genus Cucumis are very popular worldwide and have great market value. However, their fruit quality and yield are hindered by viral diseases. C. metuliferus is considered a wild species with resistance to viral diseases that is lacking in cultivated crops of the Cucumis genus, such as melon. The C. metuliferus line L37 shows extreme resistance against Papaya ringspot virus (PRSV-HA), whereas line L35 is a susceptible line. In this study, reciprocal grafting experiments between L35 and L37 were performed, and the PRSV-HA strain was pre-inoculated in the rootstock leaves. The results revealed that the resistance signal in the L37 rootstock could transmit and provide resistance to the L35 scion. Subsequently, double sandwich grafting was performed using the pre-inoculated L35 as the rootstock, which was then grafted onto the L37 intermediate and the L35 scion. The results showed that PRSV-HA RNA accumulated in the L35 rootstock leaf, petiole, and stem tissues, whereas PRSV-HA RNA accumulated in some intermediate and scion petiole and stem tissues. No HCPro RNA was detected in the L35 scion leaves. The results showed that the suppression of the virus occurred in the leaves, and the resistance effect spread from the rootstock in the scion direction. Hence, this study has demonstrated that RNA silencing of systemic signals is responsible for L37 resistance against PRSV. C. metuliferus L37 could provide a valuable resistance source for crops of the Cucumis species against viral diseases through grafting.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Melon
Plant Science
01 natural sciences
Papaya ringspot virus
Petiole (botany)
03 medical and health sciences
food
lcsh:Botany
Cucumis metuliferus
Ecology, Evolution, Behavior and Systematics
Ecology
biology
biology.organism_classification
Grafting
grafting
food.food
lcsh:QK1-989
Horticulture
RNA silencing
030104 developmental biology
virus resistance
immune
Rootstock
Cucumis
010606 plant biology & botany
Subjects
Details
- ISSN :
- 22237747
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Plants
- Accession number :
- edsair.doi.dedup.....ec71c6671d494449ed8dfd8ef774e042
- Full Text :
- https://doi.org/10.3390/plants9121666