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SERF: a new antigen in the Cromer blood group system
- Publication Year :
- 2004
- Publisher :
- Wiley, 2004.
-
Abstract
- The Cromer blood group system consists of eight high incidence and three low incidence antigens carried on decay-accelerating factor (DAF). This report describes the identification and characterization of a new Cromer high incidence antigen, named SERF. Sequence analyses of DNA from a Thai female whose serum contained the alloantibody to a high incidence antigen in the Cromer blood group system (anti-SERF) and from her two children were performed. Reverse transcriptase-polymerase chain reaction (RT-PCR) and sequence analysis on cDNA from the proband and PCR-restriction fragment length polymorphism analysis on DNA from Thais were also performed. To map the epitope, DAF deletion mutants were tested by immunoblotting with anti-SERF. Sequence analysis revealed a substitution of 647C>T in exon 5 DAF in the proband. The proband's two children and two of 100 Thais were heterozygotes 647C/T. Analysis using DAF deletion mutants revealed the antigenic determinant to be within short consensus repeat 3 (SCR3), which is encoded by exon 5. This study describes a novel high incidence antigen (SERF) in the Cromer blood group system characterized by the amino acid proline at position 182 in SCR3 of DAF. The SERF-negative proband has a substitution mutation that predicts for leucine at this position. SERF has been provisionally assigned the International Society of Blood Transfusion number 021��012 (CROM 12).
- Subjects :
- Proband
Genetics
Isoantigens
ddc:610
Erythrocytes
Base Sequence
Reverse Transcriptase Polymerase Chain Reaction
Sequence analysis
Point mutation
CD55, Cromer blood group, decay-accelerating factor, high incidence antigen, SERF
610 Medizin
Hematology
Biology
Molecular biology
Epitope
Exon
Antigen
Complementary DNA
Blood Group Antigens
Humans
Decay-accelerating factor
Polymorphism, Restriction Fragment Length
DNA Primers
Subjects
Details
- Language :
- German
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....ec62a9b7f3f1b126170f223e2a1d03ba