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P53 regulation of leukemia cells with the blockage of MDM2 by antisense oligonucleotides
- Source :
- Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban. 26(4)
- Publication Year :
- 2006
-
Abstract
- The changes of expression and function of MDM2 and P53 by MDM2 specific antisense oligonucleotides were investigated in HL60 cells. Cells were divided into control group, AS group (MDM2 specific antisense oligonucleotides group), cisplatin group, and combined treatment group. FCM analysis and Western blot and RT-PCR were used to estimate apoptosis and the expression of MDM2 and P53. Our results showed that the transfection of MDM2 specific antisense oligonucleotides obviously inhibited MDM2 expression (P
- Subjects :
- Genetic enhancement
Blotting, Western
Biomedical Engineering
Apoptosis
HL-60 Cells
Transfection
Biochemistry
Biomaterials
Western blot
Genetics
medicine
Humans
neoplasms
Cisplatin
Oncogene
medicine.diagnostic_test
Chemistry
Oligonucleotide
Reverse Transcriptase Polymerase Chain Reaction
Proto-Oncogene Proteins c-mdm2
Oligonucleotides, Antisense
medicine.disease
Flow Cytometry
Molecular biology
Gene Expression Regulation, Neoplastic
enzymes and coenzymes (carbohydrates)
Leukemia
Cancer research
Tumor Suppressor Protein p53
medicine.drug
Subjects
Details
- ISSN :
- 16720733
- Volume :
- 26
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban
- Accession number :
- edsair.doi.dedup.....ec5a69ba8f709ef18e638c3212824291