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Loss of membrane asymmetry alters the interactions of erythrocytes with engineered silica nanoparticles
- Source :
- Biointerphases
- Publication Year :
- 2020
- Publisher :
- American Vacuum Society, 2020.
-
Abstract
- Disruption of plasma membrane integrity is a primary mechanism of nanoparticle toxicity in cells. Mechanistic studies on nanoparticle-induced membrane damage have been commonly performed using model membranes with a focus on symmetric bilayers, overlooking the fact that the membrane has an asymmetric phospholipid composition. In this study, erythrocytes with normal and scrambled membrane asymmetry were utilized to examine how the loss of membrane asymmetry and the resulting alterations in the outer leaflet lipid composition affect nanoparticle-membrane interactions. Unmodified, amine-modified, and carboxyl-modified silica (30 nm) were used as nanoparticle models. Loss of membrane asymmetry was achieved by induction of eryptosis, using a calcium ionophore. Erythrocyte membrane disruption (hemolysis) by unmodified silica nanoparticles was significantly reduced in eryptotic compared to healthy cells. Amine- and carboxyl-modified particles did not cause hemolysis in either cell. In agreement, a significant reduction in the binding of unmodified silica nanoparticles to the membrane was observed upon loss of membrane asymmetry. Unmodified silica particles also caused significant cell deformation, changing healthy erythrocytes into a spheroid shape. In agreement with findings in the cells, unmodified particles disrupted vesicles mimicking the erythrocyte outer leaflet lipid composition. The degree of disruption and nanoparticle binding to the membrane was reduced in vesicles mimicking the composition of scrambled membranes. Cryo-electron microscopy revealed the presence of lipid layers on particle surfaces, pointing to lipid adsorption as the mechanism for vesicle damage. Together, findings indicate an important role for the lipid composition of the membrane outer leaflet in nanoparticle-induced membrane damage in both vesicles and erythrocytes.
- Subjects :
- Erythrocytes
Cell
Eryptosis
Phospholipid
Ionophore
General Physics and Astronomy
chemistry.chemical_element
Nanoparticle
02 engineering and technology
Calcium
010402 general chemistry
Hemolysis
01 natural sciences
General Biochemistry, Genetics and Molecular Biology
Biomaterials
chemistry.chemical_compound
medicine
Humans
General Materials Science
Amines
Chemistry
Vesicle
Cell Membrane
Cryoelectron Microscopy
Articles
General Chemistry
Silicon Dioxide
021001 nanoscience & nanotechnology
medicine.disease
0104 chemical sciences
Membrane
medicine.anatomical_structure
Biophysics
Nanoparticles
0210 nano-technology
Subjects
Details
- ISSN :
- 15594106 and 19348630
- Volume :
- 15
- Database :
- OpenAIRE
- Journal :
- Biointerphases
- Accession number :
- edsair.doi.dedup.....ec591955ac847b3c25eb6380f1153432
- Full Text :
- https://doi.org/10.1116/6.0000246