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Comparative assessment of fermentative capacity of different xylose-consuming yeasts
- Source :
- Microbial Cell Factories, Vol 16, Iss 1, Pp 1-8 (2017), Microbial Cell Factories
- Publication Year :
- 2017
- Publisher :
- Springer Science and Business Media LLC, 2017.
-
Abstract
- Background Understanding the effects of oxygen levels on yeast xylose metabolism would benefit ethanol production. In this work, xylose fermentative capacity of Scheffersomyces stipitis, Spathaspora passalidarum, Spathaspora arborariae and Candida tenuis was systematically compared under aerobic, oxygen-limited and anaerobic conditions. Results Fermentative performances of the four yeasts were greatly influenced by oxygen availability. S. stipitis and S. passalidarum showed the highest ethanol yields (above 0.44 g g−1) under oxygen limitation. However, S. passalidarum produced 1.5 times more ethanol than S. stipitis under anaerobiosis. While C. tenuis showed the lowest xylose consumption rate and incapacity to produce ethanol, S. arborariae showed an intermediate fermentative performance among the yeasts. NAD(P)H xylose reductase (XR) activity in crude cell extracts correlated with xylose consumption rates and ethanol production. Conclusions Overall, the present work demonstrates that the availability of oxygen influences the production of ethanol by yeasts and indicates that the NADH-dependent XR activity is a limiting step on the xylose metabolism. S. stipitis and S. passalidarum have the greatest potential for ethanol production from xylose. Both yeasts showed similar ethanol yields near theoretical under oxygen-limited condition. Besides that, S. passalidarum showed the best xylose consumption and ethanol production under anaerobiosis.
- Subjects :
- 0301 basic medicine
lcsh:QR1-502
chemistry.chemical_element
Bioethanol
Bioengineering
Xylose
Biology
Oxygen availability
Applied Microbiology and Biotechnology
Oxygen
lcsh:Microbiology
03 medical and health sciences
chemistry.chemical_compound
Xylose metabolism
Aldehyde Reductase
Ethanol fuel
Anaerobiosis
Ethanol
Research
Xylose reductase
Yeast
030104 developmental biology
chemistry
Biochemistry
Biofuel
Fermentation
Saccharomycetales
Xylose fermentation
Yeast fermentation
Anaerobic exercise
Biotechnology
Subjects
Details
- ISSN :
- 14752859
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- Microbial Cell Factories
- Accession number :
- edsair.doi.dedup.....ec3f5d4ccc2c27bbb1e6508e4d2d6a60