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Shootin1: a protein involved in the organization of an asymmetric signal for neuronal polarization
- Source :
- Toriyama, M, Shimada, T, Kim, K B, Mitsuba, M, Nomura, E, Katsuta, K, Sakumura, Y, Roepstorff, P & Inagaki, N 2006, ' Shootin1 : A protein involved in the organization of an asymmetric signal for neuronal polarization ', Journal of Cell Biology, vol. 175, no. 1, pp. 147-157 . https://doi.org/10.1083/jcb.200604160, The Journal of Cell Biology
- Publication Year :
- 2006
- Publisher :
- The Rockefeller University Press, 2006.
-
Abstract
- Neurons have the remarkable ability to polarize even in symmetrical in vitro environments. Although recent studies have shown that asymmetric intracellular signals can induce neuronal polarization, it remains unclear how these polarized signals are organized without asymmetric cues. We describe a novel protein, named shootin1, that became up-regulated during polarization of hippocampal neurons and began fluctuating accumulation among multiple neurites. Eventually, shootin1 accumulated asymmetrically in a single neurite, which led to axon induction for polarization. Disturbing the asymmetric organization of shootin1 by excess shootin1 disrupted polarization, whereas repressing shootin1 expression inhibited polarization. Overexpression and RNA interference data suggest that shootin1 is required for spatially localized phosphoinositide-3-kinase activity. Shootin1 was transported anterogradely to the growth cones and diffused back to the soma; inhibiting this transport prevented its asymmetric accumulation in neurons. We propose that shootin1 is involved in the generation of internal asymmetric signals required for neuronal polarization.
- Subjects :
- Proteomics
Neurite
Growth Cones
Molecular Sequence Data
Nerve Tissue Proteins
Hippocampal formation
Biology
Hippocampus
Models, Biological
Article
Phosphatidylinositol 3-Kinases
Cell polarity
medicine
Animals
Amino Acid Sequence
Axon
Growth cone
Research Articles
Neurons
Cell Polarity
Cell Biology
Rats
Transport protein
Cell biology
Protein Transport
medicine.anatomical_structure
Gene Expression Regulation
Soma
Sequence Alignment
Intracellular
Subjects
Details
- Language :
- English
- ISSN :
- 15408140
- Volume :
- 175
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- The Journal of Cell Biology
- Accession number :
- edsair.doi.dedup.....ec2fa5ac1fd9e086837b021cdef3f190
- Full Text :
- https://doi.org/10.1083/jcb.200604160