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Shootin1: a protein involved in the organization of an asymmetric signal for neuronal polarization

Authors :
Kazuhiro Katsuta
Peter Roepstorff
Mari Mitsuba
Yuichi Sakumura
Naoyuki Inagaki
Tadayuki Shimada
Ki Bum Kim
Eiko Nomura
Michinori Toriyama
Source :
Toriyama, M, Shimada, T, Kim, K B, Mitsuba, M, Nomura, E, Katsuta, K, Sakumura, Y, Roepstorff, P & Inagaki, N 2006, ' Shootin1 : A protein involved in the organization of an asymmetric signal for neuronal polarization ', Journal of Cell Biology, vol. 175, no. 1, pp. 147-157 . https://doi.org/10.1083/jcb.200604160, The Journal of Cell Biology
Publication Year :
2006
Publisher :
The Rockefeller University Press, 2006.

Abstract

Neurons have the remarkable ability to polarize even in symmetrical in vitro environments. Although recent studies have shown that asymmetric intracellular signals can induce neuronal polarization, it remains unclear how these polarized signals are organized without asymmetric cues. We describe a novel protein, named shootin1, that became up-regulated during polarization of hippocampal neurons and began fluctuating accumulation among multiple neurites. Eventually, shootin1 accumulated asymmetrically in a single neurite, which led to axon induction for polarization. Disturbing the asymmetric organization of shootin1 by excess shootin1 disrupted polarization, whereas repressing shootin1 expression inhibited polarization. Overexpression and RNA interference data suggest that shootin1 is required for spatially localized phosphoinositide-3-kinase activity. Shootin1 was transported anterogradely to the growth cones and diffused back to the soma; inhibiting this transport prevented its asymmetric accumulation in neurons. We propose that shootin1 is involved in the generation of internal asymmetric signals required for neuronal polarization.

Details

Language :
English
ISSN :
15408140
Volume :
175
Issue :
1
Database :
OpenAIRE
Journal :
The Journal of Cell Biology
Accession number :
edsair.doi.dedup.....ec2fa5ac1fd9e086837b021cdef3f190
Full Text :
https://doi.org/10.1083/jcb.200604160