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Low-Energy (5–20 eV) Electron-Induced Single and Double Strand Breaks in Well-Defined DNA Sequences
- Source :
- The Journal of Physical Chemistry Letters. 13:4871-4876
- Publication Year :
- 2022
- Publisher :
- American Chemical Society (ACS), 2022.
-
Abstract
- Ionizing radiation is used in cancer radiation therapy to effectively damage the DNA of tumors. The main damage is due to generation of highly reactive secondary species such as low-energy electrons (LEEs). The accurate quantification of DNA radiation damage of well-defined DNA target sequences in terms of absolute cross sections for LEE-induced DNA strand breaks is possible by the DNA origami technique; however, to date, it is possible only for DNA single strands. In the present work DNA double strand breaks in the DNA sequence 5'-d(CAC)(4)/5'd(GTG)(4) are compared with DNA single strand breaks in the oligonucleotides 5'-d(CAC)(4) and 5'-d(GTG)(4) upon irradiation with LEEs in the energy range from 5 to 20 eV. A maximum of strand break cross section was found around 7 and 10 eV independent of the DNA sequence, indicating that dissociative electron attachment is the underlying mechanism of strand breakage and confirming previous studies using plasmid DNA.
Details
- ISSN :
- 19487185
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- The Journal of Physical Chemistry Letters
- Accession number :
- edsair.doi.dedup.....ec15dc641ca57e7a9331613e9d1635ab