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Label-free electrochemical aptasensor for detection of alpha-fetoprotein based on AFP-aptamer and thionin/reduced graphene oxide/gold nanoparticles
- Source :
- Analytical Biochemistry. 547:37-44
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- Sensitive and accurate detection of tumor markers is critical to early diagnosis, point-of-care and portable medical supervision. Alpha fetoprotein (AFP) is an important clinical tumor marker for hepatocellular carcinoma (HCC), and the concentration of AFP in human serum is related to the stage of HCC. In this paper, a label-free electrochemical aptasensor for AFP detection was fabricated using AFP-aptamer as the recognition molecule and thionin/reduced graphene oxide/gold nanoparticles (TH/RGO/Au NPs) as the sensor platform. With high electrocatalytic property and large specific surface area, RGO and Au NPs were employed on the screen-printed carbon electrode to load TH molecules. The TH not only acted as a bridging molecule to effectively capture and immobilize AFP-aptamer, but as the electron transfer mediator to provide the electrochemical signal. The AFP detection was based on the monitoring of the electrochemical current response change of TH by the differential pulse voltammetry. Under optimal conditions, the electrochemical responses were proportional to the AFP concentration in the range of 0.1–100.0 μg/mL. The limit of detection was 0.050 μg/mL at a signal-to-noise ratio of 3. The proposed method may provide a promising application of aptamer with the properties of facile procedure, low cost, high selectivity in clinic.
- Subjects :
- Aptamer
Biophysics
Metal Nanoparticles
02 engineering and technology
Sensitivity and Specificity
01 natural sciences
Biochemistry
law.invention
chemistry.chemical_compound
law
Humans
Thionins
Molecular Biology
Detection limit
Graphene
010401 analytical chemistry
Cell Biology
Aptamers, Nucleotide
021001 nanoscience & nanotechnology
Combinatorial chemistry
digestive system diseases
0104 chemical sciences
Thionin
chemistry
Colloidal gold
Electrode
Graphite
Gold
alpha-Fetoproteins
Differential pulse voltammetry
0210 nano-technology
Alpha-fetoprotein
Subjects
Details
- ISSN :
- 00032697
- Volume :
- 547
- Database :
- OpenAIRE
- Journal :
- Analytical Biochemistry
- Accession number :
- edsair.doi.dedup.....ebdf96cabb556e9576a111b73e670be4