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Structure and immunomodulatory activity of polysaccharides from Fusarium solani DO7 by solid-state fermentation
- Source :
- International Journal of Biological Macromolecules. 137:568-575
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- Two polysaccharides, DGS1 and DGS2, were obtained by solid-state fermentation (SSF) of Fusarium solani DO7, an endophytic fungus isolated from the orchid Dendrobium officinale. Structural characterizations revealed that DGS1 consisted of arabinose, glucose, mannose and galactose with a molar ratio of 2.9:13.4:3.0:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp, (1 → 2,6)-Manp and (1 → 6)-Galp glycosidic linkages, while DGS2 was composed of arabinose, glucose, mannose and galactose in a molar ratio of 3.5:8.1:2.1:1, respectively, and contained (1 → 5)-Araf, (1 → 4)-Glcp, (1 → 6)-Glcp, (1 → 3)-Manp and (1 → 6)-Galp glycosidic linkages. Neither polysaccharide was toxic to human embryonic kidney cells or mouse RAW 264.7 macrophage cells. An immunomodulatory activity assay indicated that both polysaccharides could significantly enhance the levels of TNF-α, IL-6 and NO by activating TNF-α, IL-6 and iNOs gene expression, respectively, especially DGS2. Interestingly, DGS2 also possessed relatively high antioxidant activity. These results illustrate that, due to its cost-effectiveness and environmentally friendly features, SSF has significant potential as a commercially competitive source of natural products, including fungal polysaccharides, with immunomodulatory activity.
- Subjects :
- Arabinose
Glycosylation
Cell Survival
Mannose
02 engineering and technology
Polysaccharide
Methylation
Biochemistry
Antioxidants
Mice
03 medical and health sciences
chemistry.chemical_compound
Fusarium
Phagocytosis
Structural Biology
Animals
Humans
Immunologic Factors
Molecular Biology
030304 developmental biology
chemistry.chemical_classification
0303 health sciences
biology
Monosaccharides
Fungal Polysaccharides
Glycosidic bond
General Medicine
021001 nanoscience & nanotechnology
biology.organism_classification
Molecular Weight
HEK293 Cells
RAW 264.7 Cells
chemistry
Solid-state fermentation
Galactose
Fermentation
0210 nano-technology
Fusarium solani
Subjects
Details
- ISSN :
- 01418130
- Volume :
- 137
- Database :
- OpenAIRE
- Journal :
- International Journal of Biological Macromolecules
- Accession number :
- edsair.doi.dedup.....ebdb4b79131132c28e47b591b6384ee9
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2019.07.019