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Gel electrophoresis method for quantitation of gamma ray induced single- and double-strand breaks in DNA irradiatedin vitro
- Source :
- Electrophoresis. 10:318-326
- Publication Year :
- 1989
- Publisher :
- Wiley, 1989.
-
Abstract
- We describe a method based on gel electrophoresis for the quantitation of strand breaks in DNA and demonstrate its application to the measurement of single- and double-strand breaks formed by gamma-rays for DNA irradiated in vitro. For single-strand breaks, our data span the dose range from 0.1 to 1 Gy, while for double-strand breaks doses were from 3 to 15 Gy. In agreement with results obtained using other techniques, we find that the dose response function for single-strand breaks is linear while the dose response function for double-strand breaks is curved, indicating that it is the sum of both linear and quadratic components. We discuss factors that determine the sensitivity of the method and indicate approaches to make possible the quantitation of strand breaks in the DNA of cells irradiated with sublethal doses.
- Subjects :
- Electrophoresis
Clinical Biochemistry
DNA, Single-Stranded
Biology
Biochemistry
Analytical Chemistry
chemistry.chemical_compound
Photography
Irradiation
Probability
Electrophoresis, Agar Gel
Double strand
Gel electrophoresis
Gamma ray
DNA
Hydrogen-Ion Concentration
Molecular biology
In vitro
Molecular Weight
chemistry
Gamma Rays
DNA, Viral
Biophysics
T-Phages
DNA Damage
Subjects
Details
- ISSN :
- 15222683 and 01730835
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Electrophoresis
- Accession number :
- edsair.doi.dedup.....ebd4382f9533262c575daa86519a0228