Back to Search
Start Over
Liver sinusoidal endothelial cells contribute to alloreactive T-cell tolerance induced by portal venous injection of donor splenocytes
- Source :
- Transplant International. 18:237-245
- Publication Year :
- 2005
- Publisher :
- Frontiers Media SA, 2005.
-
Abstract
- We demonstrated that an indirect pathway of alloantigen presentation via liver sinusoidal endothelial cells (LSEC) is involved in alloreactive T-cell tolerance induced by portal venous injection (PI) of donor cells. Thirty million C57BL/6 (B6) splenocytes that were either untreated or treated with 30-Gy irradiation were injected via the portal vein into Balb/c mice. Host LSEC expressing major histocompatibility complex class II actively endocytosed the allogeneic naive splenocytes as well as irradiated splenocytes after PI. Using a transendothelial migration assay, it was demonstrated that host-type Balb/c CD4(+) T cells that transmigrated across LSEC that had captured irradiated B6 splenocytes were rendered tolerant to subsequent alloantigen presentation by host professional antigen-presenting cells. Consistently, PI of irradiated donor-type splenocytes led to remarkable prolongation of the survival of subsequently transplanted heart allografts. These results indicate that indirect antigen presentation by LSEC significantly contributes to alloreactive T-cell tolerance induced by PI of irradiated donor splenocytes.
- Subjects :
- Isoantigens
Cellular immunity
T-Lymphocytes
T cell
Antigen presentation
Immune tolerance
Mice
Immune system
Immune Tolerance
Splenocyte
Animals
Transplantation, Homologous
Medicine
Antigen Presentation
Mice, Inbred BALB C
Mice, Inbred C3H
Transplantation
Portal Vein
business.industry
Graft Survival
Endothelial Cells
Molecular biology
Endocytosis
Tissue Donors
Mice, Inbred C57BL
Endothelial stem cell
medicine.anatomical_structure
Lymphocyte Transfusion
Immunology
Hepatocytes
Heart Transplantation
Female
business
Spleen
Subjects
Details
- ISSN :
- 14322277 and 09340874
- Volume :
- 18
- Database :
- OpenAIRE
- Journal :
- Transplant International
- Accession number :
- edsair.doi.dedup.....ebd1cc6c04874096687f6b6ee6c452f4