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Characterization of the insulinotropic action of a phospholipase A2 isolated from Crotalus durissus collilineatus rattlesnake venom on rat pancreatic islets
- Publication Year :
- 2005
- Publisher :
- Elsevier, 2005.
-
Abstract
- The ability of PLA2 and crotapotin, isolated from Crotalus durissus collilineatus rattlesnake venom, to stimulate insulin secretion from isolated rat islets was examined. PLA2 and crotapotin stimulated insulin secretion at 2.8 mmol/L glucose, whereas at a high glucose concentrations (16.7 mmol/L) only PLA2 stimulated secretion. Nifedipine (10 micromol/L) did not alter the ability of PLA2 to increase insulin secretion stimulated by a depolarizing concentration of K+ (30 mmol/L). PLA2 did not affect 14CO2 production but significantly increased the efflux of arachidonic acid from isolated islets. These results indicate that PLA2-stimulated secretion is not dependent on an additional influx of Ca2+ through L-type Ca(2+)-channels but rather is associated with arachidonic acid formation in pancreatic islets. ispartof: Toxicon vol:45 issue:2 pages:243-8 ispartof: location:England status: published
- Subjects :
- Male
medicine.medical_specialty
medicine.medical_treatment
In Vitro Techniques
Toxicology
Phospholipases A
chemistry.chemical_compound
Islets of Langerhans
Phospholipase A2
Internal medicine
Insulin Secretion
Crotalid Venoms
medicine
Animals
Insulin
Secretion
Rats, Wistar
Arachidonic Acid
biology
Pancreatic islets
Crotalus
biology.organism_classification
Crotoxin
Rats
Phospholipases A2
Endocrinology
medicine.anatomical_structure
L-Glucose
chemistry
Snake venom
biology.protein
Arachidonic acid
lipids (amino acids, peptides, and proteins)
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....ebce61f5ba8ef2037aff51fbcaac8878