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Urinary metabolomics reveals the therapeutic effect of HuangQi Injections in cisplatin-induced nephrotoxic rats
- Source :
- Scientific Reports, Scientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
- Publication Year :
- 2017
- Publisher :
- Nature Publishing Group UK, 2017.
-
Abstract
- The side effects of cisplatin (CDDP), notably nephrotoxicity, greatly limited its use in clinical chemotherapy. HuangQi Injections (HI), a commonly used preparation of the well-known Chinese herbal medicine Astragali radix, appeared to be promising treatment for nephrotoxicity without compromising the anti-tumor activity of CDDP. In this study, the urinary metabolomics approach using liquid chromatography time of flight mass spectrometry (LC-TOF/MS) was developed to assess the toxicity-attenuation effects and corresponding mechanisms of HI on CDDP-exposed rats. As a result, successive administration of HI significantly recovered the decline of body weight and downregulated the abnormal increase of serum creatinine and urea. HI partly restored the CDDP-induced alteration of metabolic profiling back into normal condition. Totally 43 toxicity-attenuation potential biomarkers were screened and tentatively identified, which were involved in important metabolic pathways such as amino acid metabolism, TCA cycle, fatty acid metabolism, vitamin B6 metabolism and purine metabolism. The results clearly revealed that HI could alleviate CDDP-induced nephrotoxicity and improve the disturbed metabolic balance induced by repeated CDDP exposure. The present study provided reliable evidence for the protective effect of HI on CDDP-induced toxicity with the multi-target pharmacological characteristics.
- Subjects :
- 0301 basic medicine
inorganic chemicals
Male
Science
Antineoplastic Agents
Pharmacology
Article
Nephrotoxicity
03 medical and health sciences
chemistry.chemical_compound
Tandem Mass Spectrometry
medicine
Metabolome
Animals
Metabolomics
Purine metabolism
Cisplatin
Creatinine
Multidisciplinary
Fatty acid metabolism
business.industry
Metabolism
Rats
Disease Models, Animal
030104 developmental biology
chemistry
Toxicity
Medicine
Kidney Diseases
business
Biomarkers
Metabolic Networks and Pathways
medicine.drug
Chromatography, Liquid
Drugs, Chinese Herbal
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....ebc27328e150752da425989cdf79c9f8