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Folate-conjugated thermo-responsive micelles for tumor targeting
- Source :
- Journal of Biomedical Materials Research Part A. :3134-3142
- Publication Year :
- 2012
- Publisher :
- Wiley, 2012.
-
Abstract
- Folate-conjugated and thermo-responsive poly((N-isopropylacrylamide)-co- acrylamide-co-(octadecyl acrylate)-co-(folate-(polyethylene glycol)-(acrylic acid))) (P(NIPA-co-AAm-co-ODA-co-FPA)) micelles with mean diameter of about 60 nm and lower critical solution temperature (LCST) of about 39°C were synthesized by free radical random copolymerization. Single-factor tests of acrylamide and octadecyl acrylate were carried out to modulate micelles' LCST and diameter, respectively. LCST, diameter, and morphology of micelles were determined by UV-vis spectrophotometer, laser particle size analyzer, and transmittance electron microscope (TEM), respectively. Fluorescein was then used as a model drug to investigate the drug loading content of micelles. Micelles with maximum amount of octadecyl acrylate (180 mg) were found to yield drug loading content of 10.48%. Near infrared dye No.10 was chosen as the tracer to monitor micelles in vivo. The targeting behaviors of micelles in folate receptor positive Bel-7402 tumor bearing nude mice were assessed by a self-constructed near infrared imaging system. Results showed satisfactory targeting capability of the thermo-responsive micelles toward Bel-7402 tumors, and targeting accumulation could last for more than 96 h, enabling P(NIPA-co-AAm-co-ODA-co-FPA) micelles to function as a diagnostic reagent as well as a targeted tumor therapy.
- Subjects :
- Male
Materials science
Biomedical Engineering
Mice, Nude
Polyethylene glycol
Lower critical solution temperature
Micelle
Cell Line
Biomaterials
Mice
chemistry.chemical_compound
Drug Delivery Systems
Folic Acid
Neoplasms
Polymer chemistry
Copolymer
Animals
Humans
Fluorescein
Micelles
Acrylic acid
Acrylamide
Acrylate
Folate Receptors, GPI-Anchored
Temperature
Metals and Alloys
Acrylates
chemistry
Folate receptor
Delayed-Action Preparations
Ceramics and Composites
Female
Nuclear chemistry
Subjects
Details
- ISSN :
- 15493296
- Database :
- OpenAIRE
- Journal :
- Journal of Biomedical Materials Research Part A
- Accession number :
- edsair.doi.dedup.....ebb4da9cf4ced75c042fa07c2908d33d