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Tuboperitoneal fistula, ectopic pregnancy, and remnants of fallopian tube: a confocal microtomography analysis and 3D reconstruction of human fallopian tube pathologies
- Source :
- The Journal of Maternal-Fetal & Neonatal Medicine. 32:3082-3087
- Publication Year :
- 2018
- Publisher :
- Informa UK Limited, 2018.
-
Abstract
- Objective: The aim of this study was to provide a three-dimensional (3D) microscopic reconstruction of morphological modifications of the fallopian tube (FT) following surgical sterilization (including tuboperitoneal fistula) and ectopic pregnancy (EP) using confocal microtomography (micro-CT). Methods: Six specimens of FT from elective and emergency surgeries were selected: three remnants of the FT (RFT) from surgical sterilization, including one presenting tuboperitoneal fistula, and three FTs with EP. The specimens were fixed in formalin and stained with Lugol solution. Micro-CT studies were conducted on the specimens using protocols adapted from biological studies. Results: Three RFTs from surgical sterilization and three FTs affected by EPs were successfully scanned using micro-CT. There was good contrast impregnation, allowing tissue segmentation and analysis of different sections of the FTs. Three FT specimens from EP exhibited considerable distortion of the tubal anatomy, mainly from the blood clot in the tubal abortion. Three RFT specimens exhibited some features observed in traditional microscopy, such as tubal distension and loss of normal anatomical characteristics of a normal FT, and indicated the presence of a tuboperitoneal fistula in one of the three specimens. Conclusion: Micro-CT can identify morphological characteristics of FT pathologies previously described in a microscopic scale, with tissue contrast and the possibility of 3D reconstruction. Micro-CT is also useful in guiding traditional sectioning of specimens for histopathological studies.
- Subjects :
- Fistula
Confocal
Surgical sterilization
Peritoneal Diseases
03 medical and health sciences
Imaging, Three-Dimensional
0302 clinical medicine
Pregnancy
medicine
Humans
Fallopian Tubes
Ultrasonography
030219 obstetrics & reproductive medicine
Biological studies
Tissue segmentation
Ectopic pregnancy
business.industry
Obstetrics and Gynecology
X-Ray Microtomography
medicine.disease
medicine.anatomical_structure
030220 oncology & carcinogenesis
Pediatrics, Perinatology and Child Health
Female
Pregnancy, Tubal
Nuclear medicine
business
Fallopian tube
Subjects
Details
- ISSN :
- 14764954 and 14767058
- Volume :
- 32
- Database :
- OpenAIRE
- Journal :
- The Journal of Maternal-Fetal & Neonatal Medicine
- Accession number :
- edsair.doi.dedup.....eb8497ba9ab21878adb4a774c96b2aeb