Back to Search
Start Over
Analysis of the efficiency of the rabbit whey acidic protein gene 5′ flanking region in controlling the expression of homologous and heterologous linked genes
- Source :
- Digital.CSIC. Repositorio Institucional del CSIC, instname
- Publication Year :
- 2005
- Publisher :
- Cambridge University Press (CUP), 2005.
-
Abstract
- 7 pages.-- PMID: 16180729 [PubMed].<br />For 10 years, the regulatory regions of the mouse and rabbit whey acidic protein gene have been used to express heterologous proteins in the milk of transgenic mice, as well as to produce pharmaceutical proteins, on a large scale, in the milk of transgenic livestock. To date, a broad range of expression levels have been detected, and elucidation of the structure-function relationship in these regulatory regions might help to achieve high levels of expression, reproducibly. An extended 5′ regulatory region (17·6 kb v. 6·3 kb) of the rabbit whey acidic promoter resulted in an increased frequency of rabbit whey acidic protein expression in transgenic mice. However, the expression levels were low compared with the high expression levels achieved in both transgenic mice and rabbits using the heterologous κ-casein in the 6·3 kb rabbit whey acidic protein 5′ regulatory region. These results underline the importance of the 3’ downstream regulatory regions, which still need to be better characterized in the whey acidic protein gene.
- Subjects :
- Whey protein
Livestock
Mouse
Genetic Linkage
Transgene
Mammary gland
5' flanking region
Heterologous
Mice, Transgenic
Rabbit
Regulatory Sequences, Nucleic Acid
Whey acidic protein
Transfection
Mice
Animals
Promoter Regions, Genetic
Gene
Regulatory regions
Regulation of gene expression
biology
Transgenic animals
Caseins
food and beverages
General Medicine
Milk Proteins
Milk
Gene Expression Regulation
Biochemistry
Regulatory sequence
biology.protein
Protein expression
Rat
Animal Science and Zoology
Rabbits
Whey Acidic Protein
Food Science
Subjects
Details
- ISSN :
- 14697629 and 00220299
- Volume :
- 72
- Database :
- OpenAIRE
- Journal :
- Journal of Dairy Research
- Accession number :
- edsair.doi.dedup.....eb7fe83c7b8d9db9229765b39ba8145c