Multicolor far-field fluorescence nanoscopy through isolated detection of distinct molecular species

By combining the photoswitching and localization of individual fluorophores with spectroscopy on the single molecule level, we demonstrate simultaneous multicolor imaging with low crosstalk and down to 15 nm spatial resolution using only two detection color channels. The applicability of the method to biological specimens is demonstrated on mammalian cells. The combination of far-field fluorescence nanoscopy with the recording of a single switchable molecular species at a time opens up a new class of functional imaging techniques. Fil: Bossi, Mariano Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina Fil: Folling, Jonas. Max Planck Institute for Biophysical Chemistry; Alemania Fil: Belov, Vladimir N.. Max Planck Institute for Biophysical Chemistry; Alemania Fil: Boyarskiy, Vadim P.. Max Planck Institute for Biophysical Chemistry; Alemania Fil: Medda, Rebecca. Max Planck Institute for Biophysical Chemistry; Alemania Fil: Egner, Alexander. Max Planck Institute for Biophysical Chemistry; Alemania Fil: Eggeling, Christian. Max Planck Institute for Biophysical Chemistry; Alemania Fil: Schonle, Andreas. Max Planck Institute for Biophysical Chemistry; Alemania Fil: Hell, Stefan W.. Max Planck Institute for Biophysical Chemistry; Alemania