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Multicolor far-field fluorescence nanoscopy through isolated detection of distinct molecular species
- Source :
- Nano Lett. 2008;8(8):2463-2468, Biblioteca Digital (UBA-FCEN), Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales, instacron:UBA-FCEN, Nano Letters
- Publication Year :
- 2008
-
Abstract
- By combining the photoswitching and localization of individual fluorophores with spectroscopy on the single molecule level, we demonstrate simultaneous multicolor imaging with low crosstalk and down to 15 nm spatial resolution using only two detection color channels. The applicability of the method to biological specimens is demonstrated on mammalian cells. The combination of far-field fluorescence nanoscopy with the recording of a single switchable molecular species at a time opens up a new class of functional imaging techniques. Fil: Bossi, Mariano Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina Fil: Folling, Jonas. Max Planck Institute for Biophysical Chemistry; Alemania Fil: Belov, Vladimir N.. Max Planck Institute for Biophysical Chemistry; Alemania Fil: Boyarskiy, Vadim P.. Max Planck Institute for Biophysical Chemistry; Alemania Fil: Medda, Rebecca. Max Planck Institute for Biophysical Chemistry; Alemania Fil: Egner, Alexander. Max Planck Institute for Biophysical Chemistry; Alemania Fil: Eggeling, Christian. Max Planck Institute for Biophysical Chemistry; Alemania Fil: Schonle, Andreas. Max Planck Institute for Biophysical Chemistry; Alemania Fil: Hell, Stefan W.. Max Planck Institute for Biophysical Chemistry; Alemania
- Subjects :
- Físico-Química, Ciencia de los Polímeros, Electroquímica
Imaging techniques
Single-molecule levels
chemistry.chemical_compound
Spatial resolutions
animal
General Materials Science
Far fields
fluorescence nanoscopy
Image resolution
Mammals
Microscopy
Molecular Structure
Ciencias Químicas
article
Mammalian cells
methodology
Condensed Matter Physics
Multicolor imaging
Fluorescence
Crosstalk (biology)
Biological specimens
Color channels
single molecules
Functional imaging
CIENCIAS NATURALES Y EXACTAS
Fluorophore
New class
Color
Bioengineering
Nanotechnology
Near and far field
chemistry
Cell Line
Biological specimen
rhodamines
Optical switches
Animals
Spectroscopy
Fluorescent Dyes
Mechanical Engineering
Molecular species
Switchable
General Chemistry
Photoswitching
chemical structure
Biophysics
fluorescent dye
Subjects
Details
- ISSN :
- 15306984
- Volume :
- 8
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Nano letters
- Accession number :
- edsair.doi.dedup.....eb696a9cc4ad9e567bdc9f3b39dbfdeb