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In vitro Dermal Absorption: Sample Application and Seal Quality in a Franz Diffusion Cell System
- Source :
- Skin Pharmacology and Physiology. 28:245-249
- Publication Year :
- 2015
- Publisher :
- S. Karger AG, 2015.
-
Abstract
- One of the known drawbacks of in vitro dermal absorption methods is their high interlaboratory variation. Although often attributed to biological skin differences, it has been shown that validation of other parameters such as temperature and stirring speed can reduce the high variability observed. The Organisation for Economic Co-operation and Development (OECD) and, at the EU level, the Scientific Committee on Consumer Safety (SCCS) have published guidance documents of how to perform these in vitro tests. For the parameter ‘sample application' and ‘adequate seal', it is indicated to apply the sample homogeneously and provide an adequate seal between the donor chamber and the membrane on which the sample is applied. Here, a simple and visual densitometer-based method is provided, which makes evaluation possible of any application protocol used.
- Subjects :
- Materials science
Franz diffusion cell
Physiology
Skin Absorption
media_common.quotation_subject
Sample (material)
High variability
Dermatology
In Vitro Techniques
Administration, Cutaneous
Seal (mechanical)
Diffusion
Application protocol
Dermal absorption, in vitro
Humans
Quality (business)
Densitometer
Skin
media_common
Pharmacology
cosmetics
Percutaneous absorption
Temperature
Guidance documents
General Medicine
Diffusion Chambers, Culture
Densitometry
Biomedical engineering
Subjects
Details
- ISSN :
- 16605535 and 16605527
- Volume :
- 28
- Database :
- OpenAIRE
- Journal :
- Skin Pharmacology and Physiology
- Accession number :
- edsair.doi.dedup.....eb68c67b5e1590630991133cfa8900ff