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3,4-Methylenedioxymethamphetamine (MDMA, ecstasy)-induced egr-1 mRNA in rat brain: pharmacological manipulation
- Source :
- European Journal of Pharmacology. 402:215-222
- Publication Year :
- 2000
- Publisher :
- Elsevier BV, 2000.
-
Abstract
- Using in situ hybridization and immunohistochemical techniques, we examined the expression pattern of egr-1 mRNA and Egr-1 protein in several brain regions following administration of 3,4-methylenedioxymethamphetamine (MDMA). Furthermore, we also studied the role of N -methyl- d -aspartate (NMDA) receptor, dopamine D 1 receptor, 5-hydroxytryptamine (5-HT) transporter or 5-HT 2A receptor in the induction of egr-1 mRNA by MDMA. Basal constitutive levels of egr-1 mRNA were detected in control rat brains. A single administration of MDMA (10 mg/kg) caused marked induction of egr-1 mRNA in the prefrontal cortex, striatum and hippocampal dentate gyrus. However, no changes in the egr-1 mRNA levels were detected in the CA1 region of hippocampus and occipital cortex after administration of MDMA (10 mg/kg). Furthermore, the expression of egr-1 mRNA in the prefrontal cortex, striatum and hippocampal dentate gyrus after administration of MDMA (10 mg/kg) was blocked significantly by pretreatment with NMDA receptor antagonist (5R,10S)-(+)-5-methyl-10,11-dihydro-5H-dibenzo[a,b]-cyclohepten-5,10-imine ((+)-MK801; 1 mg/kg), dopamine D 1 receptor antagonist SCH 23390 (1 mg/kg) or 5-HT uptake inhibitor paroxetine (5 mg/kg), but not by 5-HT 2A receptor antagonist SR46349B (5 mg/kg). However, high basal levels of Egr-1 immunoreactivity in the rat brain were not altered by administration of MDMA (10 mg/kg). These results suggest that MDMA alters the expression of egr-1 mRNA in several regions of rat brain, and that the expression of egr-1 mRNA by MDMA in the prefrontal cortex, striatum and hippocampal dentate gyrus appears to be mediated, at least in part, by NMDA receptor, dopamine D 1 receptor and 5-HT transporter.
- Subjects :
- Male
medicine.drug_class
N-Methyl-3,4-methylenedioxyamphetamine
Hippocampus
Pharmacology
Hippocampal formation
Receptors, N-Methyl-D-Aspartate
Immediate-Early Proteins
Rats, Sprague-Dawley
chemistry.chemical_compound
Phenols
Dopamine
medicine
Animals
RNA, Messenger
In Situ Hybridization
Early Growth Response Protein 1
Brain Chemistry
SCH-23390
Chemistry
Receptors, Dopamine D1
Dentate gyrus
MDMA
Benzazepines
Receptor antagonist
Immunohistochemistry
Rats
DNA-Binding Proteins
Fluorobenzenes
body regions
Paroxetine
Hallucinogens
Dopamine Antagonists
NMDA receptor
Serotonin Antagonists
Dizocilpine Maleate
Excitatory Amino Acid Antagonists
Selective Serotonin Reuptake Inhibitors
hormones, hormone substitutes, and hormone antagonists
Transcription Factors
medicine.drug
Subjects
Details
- ISSN :
- 00142999
- Volume :
- 402
- Database :
- OpenAIRE
- Journal :
- European Journal of Pharmacology
- Accession number :
- edsair.doi.dedup.....eb5c845d96d21d223ff5d5ca1a8415cc