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Retromer maintains basolateral distribution of the type II TGF-β receptor via the recycling endosome
- Source :
- Molecular Biology of the Cell
- Publication Year :
- 2013
- Publisher :
- American Society for Cell Biology (ASCB), 2013.
-
Abstract
- After basolateral (BL) cell surface delivery, retromer promotes type II TGF-β receptor exit and recycling to the BL plasma membrane. In the absence of retromer, however, type II receptors aberrantly sort and are mislocalized such that both BL and apical expression is observed independent of the Rab11-positive apical recycling endosome.<br />Transforming growth factor β (TGF-β) is critical for the development and maintenance of epithelial structures. Because receptor localization and trafficking affect the cellular and organismal response to TGF-β, the present study was designed to address how such homeostatic control is regulated. To that end, we identify a new role for the mammalian retromer complex in maintaining basolateral plasma membrane expression of the type II TGF-β receptor (TβRII). Retromer and TβRII associate in the presence or absence of TGF-β ligand. After retromer knockdown, although TβRII internalization and trafficking to a Rab5-positive compartment occur as in wild-type cells, receptor recycling is inhibited. This results in TβRII mislocalization from the basolateral to both the basolateral and apical plasma membranes independent of Golgi transit and the Rab11-positive apical recycling endosome. The data support a model in which, after initial basolateral TβRII delivery, steady-state polarized TβRII expression is maintained by retromer/TβRII binding and delivery to the common recycling endosome.
- Subjects :
- Receptor recycling
Retromer
Endosome
Vesicular Transport Proteins
Endosomes
Protein Serine-Threonine Kinases
Biology
Madin Darby Canine Kidney Cells
symbols.namesake
Dogs
Transforming Growth Factor beta
Cell polarity
Animals
Humans
Molecular Biology
rab5 GTP-Binding Proteins
Cell Membrane
Receptor, Transforming Growth Factor-beta Type II
Cell Polarity
Articles
Cell Biology
Basolateral plasma membrane
Golgi apparatus
Apical recycling endosome
Cell biology
Retromer complex
Protein Transport
Gene Expression Regulation
Membrane Trafficking
rab GTP-Binding Proteins
symbols
Receptors, Transforming Growth Factor beta
Signal Transduction
Subjects
Details
- ISSN :
- 19394586 and 10591524
- Volume :
- 24
- Database :
- OpenAIRE
- Journal :
- Molecular Biology of the Cell
- Accession number :
- edsair.doi.dedup.....eb5468f4f3bdc7928fbb8813ec1cfec4