Initial pH-driven production of volatile fatty acid from hybrid Pennisetum

In this work, the impact of initial pH on the production of volatile fatty acids (VFAs) of hybrid Pennisetum was investigated. The batch experiments were conducted under six distinct beginning pH at a mesophilic temperature. Initial pH had an obvious effect on VFA yield and composition, and severe alkaline circumstances (pHin = 11.0) could boost VFA production and acetic acid selectivity. The highest VFAs yield and acetate proportion were obtained when the initial pH was 11.0, with 518 ± 29 mg g−1VS and 92%. Furthermore, microbial community analysis showed that alkaliphilic acetogenic anaerobe such as Amphibacillus, Tissierella, and Natronincola were the dominant species when the initial pH was 11.0. The Amphibacillus is the main hydrolysis bacterium under these conditions because of its high ability for xylan degradation at pH 9.0–10.0. Because of the increased VFA yield and superior acetic acid selectivity, the results suggest that adjusting the initial pH to 11.0 in batch mode would be possible for scaling-up purposes.