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Gene Cloning and Characterization of<scp>L</scp>-Ribulose 3-epimerase fromMesorhizobium lotiand Its Application to Rare Sugar Production
- Source :
- Bioscience, Biotechnology, and Biochemistry. 77:511-515
- Publication Year :
- 2013
- Publisher :
- Informa UK Limited, 2013.
-
Abstract
- A gene encoding L-ribulose 3-epimerase (L-RE) from Mesorhizobium loti, an important enzyme for rare sugar production by the Izumoring strategy, was cloned and overexpressed. The enzyme showed highest activity toward L-ribulose (230 U/mg) among keto-pentoses and keto-hexoses. This is the first report on a ketose 3-epimerase showing highest activity toward keto-pentose. The optimum enzyme reaction conditions for L-RE were determined to be sodium phosphate buffer (pH 8.0) at 60 °C. The enzyme showed of higher maximum reaction a rate (416 U/mg) and catalytic efficiency (43 M(-1) min(-1)) for L-ribulose than other known ketose 3-epimerases. It was able to produce L-xylulose efficiently from ribitol in two-step reactions. In the end, 7.2 g of L-xylulose was obtained from 20 g of ribitol via L-ribulose at a yield of 36%.
- Subjects :
- Molecular Sequence Data
Pentoses
Ribitol
Applied Microbiology and Biotechnology
Biochemistry
Substrate Specificity
Analytical Chemistry
Xylulose
chemistry.chemical_compound
Enzyme Stability
Amino Acid Sequence
Cloning, Molecular
Molecular Biology
chemistry.chemical_classification
biology
Ribulose
Organic Chemistry
Mesorhizobium
Temperature
Ketose
General Medicine
Hydrogen-Ion Concentration
biology.organism_classification
Rare sugar
Recombinant Proteins
Mesorhizobium loti
Kinetics
Enzyme
chemistry
Metals
Carbohydrate Epimerases
Sequence Analysis
Biotechnology
Subjects
Details
- ISSN :
- 13476947 and 09168451
- Volume :
- 77
- Database :
- OpenAIRE
- Journal :
- Bioscience, Biotechnology, and Biochemistry
- Accession number :
- edsair.doi.dedup.....eb36b37246e96c1b27d7852f5fc4c30c