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Detection and Characterization of the Emerging Relapsing Fever Pathogen, Borrelia miyamotoi, from the Ixodes ricinus Tick in the Rural Trakya (Thrace) Region of Northwestern Turkey
- Source :
- Vector-Borne and Zoonotic Diseases. 16:797-799
- Publication Year :
- 2016
- Publisher :
- Mary Ann Liebert Inc, 2016.
-
Abstract
- The hard tick-borne relapsing fever agent, Borrelia miyamotoi infection in Ixodes ricinus ticks sampled from Istanbul and the countryside of Kirklareli in northwestern Turkey, was examined by TaqMan-PCR targeting 16S rDNA, nested PCR targeting 16S rDNA, the flagellin gene (flaB), and the 16S and 23S rDNA intergenic spacer (IGS), and sequencing analyses of these amplicons. B. miyamotoi was detected in 1 out of 248 I. ricinus ticks (infection rate 0.4%). The tick infected with B. miyamotoi was collected in Longos, Kirklareli province on the European side of Turkey near the Bulgarian border. The 16S rDNA, flaB, and IGS sequences from the infected tick showed high similarities to those of B. miyamotoi detected in I. ricinus in Europe.
- Subjects :
- DNA, Bacterial
0301 basic medicine
Ixodes ricinus
Turkey
relapsing fever
030231 tropical medicine
Borrelia miyamotoi
Tick
Communicable Diseases, Emerging
Microbiology
03 medical and health sciences
0302 clinical medicine
RNA, Ribosomal, 16S
Virology
Borrelia
parasitic diseases
medicine
Animals
Humans
Phylogeny
Ixodes
biology
Ricinus
Relapsing Fever
biology.organism_classification
medicine.disease
RNA, Bacterial
030104 developmental biology
Infectious Diseases
Nested polymerase chain reaction
Flagellin
Subjects
Details
- ISSN :
- 15577759 and 15303667
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- Vector-Borne and Zoonotic Diseases
- Accession number :
- edsair.doi.dedup.....eb33642efa4d51f85c1ff391c961c74a