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Rhizobium capsici sp. nov., isolated from root tumor of a green bell pepper (Capsicum annuum var. grossum) plant
- Source :
- Antonie van Leeuwenhoek. 107:773-784
- Publication Year :
- 2015
- Publisher :
- Springer Science and Business Media LLC, 2015.
-
Abstract
- A novel, Gram-staining-negative, rod-shaped, aerobic and motile bacterium, designated strain CC-SKC2(T), was isolated from the root tumor of a green bell pepper (Capsicum annuum var. grossum) plant in Taiwan. Cells were positive for oxidase and catalase activities and exhibited growth at 25-37 °C, pH 4.0-9.0 and tolerated NaCl concentrations up to 4.0 % (w/v). Strain CC-SKC2(T) is able to trigger nodulation in soybean (Glycine max Merr.), but not in Capsicum annuum var. grossum, red bean (Vigna angularis), sesbania (Sesbania roxburghii Merr.) or alfalfa (Medicago varia Martin.). The novel strain shared highest 16S rRNA gene sequence similarity to Rhizobium rhizoryzae KCTC 23652(T) and Rhizobium straminoryzae CC-LY845(T) (both 97.5 %) followed by Rhizobium lemnae L6-16(T) (97.3 %), Rhizobium pseudoryzae KCTC 23294(T) (97.1 %), and Rhizobium paknamense NBRC 109338(T) (97.0 %), whereas other Rhizobium species shared96.7 % similarity. The DNA-DNA relatedness values of strain CC-SKC2(T) with R. rhizoryzae KCTC 23652(T), R. pseudoryzae KCTC 23294(T) and R. paknamense NBRC 109338(T) were 11.4, 17.2 and 17.0 %, respectively (reciprocal values were 11.1, 28.3 and 24.0 %, respectively). Phylogenetic analysis based on 16S rRNA, atpD and recA genes revealed a distinct taxonomic position attained by strain CC-SKC2(T) with respect to other Rhizobium species. The major fatty acids in strain CC-SKC2(T) were C16:0, C19:0 cyclo ω8c, C14:0 3-OH and/or C16:1 iso I and C18:1 ω7c and/or C18:1 ω6c. The polyamine pattern showed predominance of spermidine and moderate amounts of sym-homospermidine. The predominant quinone system was ubiquinone (Q-10) and the DNA G+C content was 60.5 mol%. On the basis of polyphasic taxonomic evidence presented here, strain CC-SKC2(T) is proposed to represent a novel species within the genus Rhizobium, for which the name Rhizobium capsici sp. nov. is proposed. The type strain is CC-SKC2(T) (=BCRC 80699(T) = JCM 19535(T)).
- Subjects :
- DNA, Bacterial
Molecular Sequence Data
Taiwan
Sodium Chloride
DNA, Ribosomal
Plant Roots
Microbiology
Vigna
chemistry.chemical_compound
Cytosol
Microscopy, Electron, Transmission
RNA, Ribosomal, 16S
Sequence Homology, Nucleic Acid
Botany
Cluster Analysis
Molecular Biology
Phospholipids
Phylogeny
Base Composition
Medicago
biology
Fatty Acids
Quinones
Temperature
Nucleic Acid Hybridization
food and beverages
Sesbania
Sequence Analysis, DNA
General Medicine
Hydrogen-Ion Concentration
Ribosomal RNA
biology.organism_classification
16S ribosomal RNA
Aerobiosis
Bacterial Typing Techniques
Spermidine
Rec A Recombinases
chemistry
Rhizobium
Capsicum
Locomotion
Bacteria
Transcription Factors
Subjects
Details
- ISSN :
- 15729699 and 00036072
- Volume :
- 107
- Database :
- OpenAIRE
- Journal :
- Antonie van Leeuwenhoek
- Accession number :
- edsair.doi.dedup.....eb10f55313cc9d47c2ca5741519f2949