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Micro-computed tomography and histology to explore internal morphology in decapod larvae
- Source :
- Dipòsit Digital de la UB, Universidad de Barcelona, Digital.CSIC. Repositorio Institucional del CSIC, instname, Scientific Reports, Vol 8, Iss 1, Pp 1-11 (2018), Digibug. Repositorio Institucional de la Universidad de Granada
- Publication Year :
- 2018
- Publisher :
- Nature Publishing Group, 2018.
-
Abstract
- 11 pages, 6 figures, supplementary material https://doi.org/10.1038/s41598-018-32709-3<br />Traditionally, the internal morphology of crustacean larvae has been studied using destructive techniques such as dissection and microscopy. The present study combines advances in micro-computed tomography (micro-CT) and histology to study the internal morphology of decapod larvae, using the common spider crab (Maja brachydactyla Balss, 1922) as a model and resolving the individual limitations of these techniques. The synergy of micro-CT and histology allows the organs to be easily identified, revealing simultaneously the gross morphology (shape, size, and location) and histological organization (tissue arrangement and cell identification). Micro-CT shows mainly the exoskeleton, musculature, digestive and nervous systems, and secondarily the circulatory and respiratory systems, while histology distinguishes several cell types and confirms the organ identity. Micro-CT resolves a discrepancy in the literature regarding the nervous system of crab larvae. The major changes occur in the metamorphosis to the megalopa stage, specifically the formation of the gastric mill, the shortening of the abdominal nerve cord, the curving of the abdomen beneath the cephalothorax, and the development of functional pereiopods, pleopods, and lamellate gills. The combination of micro-CT and histology provides better results than either one alone<br />Financial support was provided by the Spanish Ministry of Economy and Competitiveness through the INIA project (grant number RTA2011-00004-00-00) to G.G. and a pre-doctoral fellowship to D.C. (FPI-INIA)
- Subjects :
- 0301 basic medicine
0106 biological sciences
Crustacean larvae
Gastric Mill
Nervous system
Gill
Male
X-ray microtomography
animal structures
Brachyura
Larves
media_common.quotation_subject
Tomografia
lcsh:Medicine
010603 evolutionary biology
01 natural sciences
03 medical and health sciences
Larvae
medicine
Animals
Sistema nerviós
Cephalothorax
Metamorphosis
lcsh:Science
Tomography
media_common
Multidisciplinary
biology
lcsh:R
Histological Techniques
Metamorphosis, Biological
Histology
Anatomy
X-Ray Microtomography
biology.organism_classification
030104 developmental biology
medicine.anatomical_structure
Larva
lcsh:Q
Female
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Dipòsit Digital de la UB, Universidad de Barcelona, Digital.CSIC. Repositorio Institucional del CSIC, instname, Scientific Reports, Vol 8, Iss 1, Pp 1-11 (2018), Digibug. Repositorio Institucional de la Universidad de Granada
- Accession number :
- edsair.doi.dedup.....eade45475e1771fdfd9aea13409e5b52