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Cloning and sequencing a novel metallothionein I isoform expressed in human reticulocytes
- Source :
- FEBS Letters. (2):210-212
- Publisher :
- Published by Elsevier B.V.
-
Abstract
- Total RNA from human reticulocytes was purified and reverse-transcribed into cDNA using an oligo-dT primer. This cDNA was used as a template for a polymerase chain reaction (PCR) with a primer specific for the N-terminal sequence of mammalian metallothioneins (MT) and a universal primer. A single amplified fragment was thus generated which when cloned and sequenced revealed two distinct MT cDNAs of almost identical molecular weights. One sequence was identical to that previously reported for human MT II and the other encoded a novel MT I isoform (MT IR). The DNA sequence of MT 1R is distinct from those documented for other MT I isoforms.
- Subjects :
- Gene isoform
Reticulocytes
Transcription, Genetic
Molecular Sequence Data
Biophysics
Biology
Biochemistry
DNA sequencing
law.invention
Reticulocyte
Structural Biology
law
Complementary DNA
Genetics
medicine
Humans
Amino Acid Sequence
Cloning, Molecular
Molecular Biology
Polymerase chain reaction
Cloning
Molecular mass
Base Sequence
Zinc status
Cell Biology
Molecular biology
Isoenzymes
medicine.anatomical_structure
Protein Biosynthesis
RNA
Metallothionein
Primer (molecular biology)
Subjects
Details
- Language :
- English
- ISSN :
- 00145793
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- FEBS Letters
- Accession number :
- edsair.doi.dedup.....eacd907cecf57e840e6b588888aa6b39
- Full Text :
- https://doi.org/10.1016/0014-5793(96)00587-X