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Convergent cascade catalyzed by monooxygenase - alcohol dehydrogenase fusion applied in organic media
- Source :
- ChemBioChem, 20(13), 1653-1658. WILEY-V C H VERLAG GMBH, Huang, L, Tang, W, Röllig, R, S. Albers, F, W. Fraaije, M & Kara, S 2019, ' Convergent cascade catalyzed by monooxygenase – alcohol dehydrogenase fusion applied in organic media ', ChemBioChem, vol. 20, no. 13, pp. 1653-1658 . https://doi.org/10.1002/cbic.201800814
- Publication Year :
- 2019
- Publisher :
- WILEY-V C H VERLAG GMBH, 2019.
-
Abstract
- With the aim of applying redox-neutral cascade reactions in organic media, fusions of a type II flavin-containing monooxygenase (FMO-E) and horse liver alcohol dehydrogenase (HLADH) were designed. The enzyme orientation and expression vector were found to influence the overall fusion enzyme activity. The resulting bifunctional enzyme retained the catalytic properties of both individual enzymes. The lyophilized cell-free extract containing the bifunctional enzyme was applied for the convergent cascade reaction consisting of cyclobutanone and butane-1,4-diol in different microaqueous media with only 5 % (v/v) aqueous buffer without any addition of external cofactor. Methyl tert-butyl ether and cyclopentyl methyl ether were found to be the best organic media for the synthesis of γ-butyrolactone, resulting in about 27 % analytical yield.
- Subjects :
- Methyl Ethers
biocatalysis
Stereochemistry
Recombinant Fusion Proteins
enzymes
Cyclopentyl methyl ether
Ether
solvent effects
Protein Engineering
010402 general chemistry
01 natural sciences
Biochemistry
Mixed Function Oxygenases
chemistry.chemical_compound
4-Butyrolactone
Cascade reaction
Escherichia coli
Animals
Rhodococcus
Phosphofructokinase 2
Horses
Molecular Biology
Alcohol dehydrogenase
chemistry.chemical_classification
biology
010405 organic chemistry
domino reactions
Organic Chemistry
Alcohol Dehydrogenase
fusion enzymes
Multifunctional Enzymes
Enzyme assay
0104 chemical sciences
Kinetics
Freeze Drying
Enzyme
chemistry
Biocatalysis
Solvents
biology.protein
Molecular Medicine
Subjects
Details
- Language :
- English
- ISSN :
- 14397633 and 14394227
- Volume :
- 20
- Issue :
- 13
- Database :
- OpenAIRE
- Journal :
- ChemBioChem
- Accession number :
- edsair.doi.dedup.....eac59a76c364130faecc257f29e9e032