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Mitochondrial creatine kinase and mitochondrial outer membrane porin show a direct interaction that is modulated by calcium
- Source :
- Journal of Biological Chemistry, Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2001, 276 (51), pp.48027-30. ⟨10.1074/jbc.M106524200⟩
- Publication Year :
- 2001
- Publisher :
- HAL CCSD, 2001.
-
Abstract
- International audience; Mitochondrial creatine kinase (MtCK) co-localizes with mitochondrial porin (voltage-dependent anion channel) and adenine nucleotide translocator in mitochondrial contact sites. A specific, direct protein-protein interaction between MtCK and mitochondrial porin was demonstrated using surface plasmon resonance spectroscopy. This interaction was independent of the immobilized binding partner (porin reconstituted in liposomes or MtCK) or the analyzed isoform (chicken sarcomeric MtCK or human ubiquitous MtCK, human recombinant porin, or purified bovine porin). Increased ionic strength reduced the binding of MtCK to porin, suggesting predominantly ionic interactions. By contrast, micromolar concentrations of Ca(2+) increased the amount of bound MtCK, indicating a physiological regulation of complex formation. No interaction of MtCK with reconstituted adenine nucleotide translocator was detectable in our experimental setup. The relevance of these findings for structure and function of mitochondrial contact sites is discussed.
- Subjects :
- Gene isoform
MESH: Mitochondria
chemistry.chemical_element
Porins
MESH: Microscopy, Electron
Biology
Calcium
Biochemistry
law.invention
03 medical and health sciences
0302 clinical medicine
law
[SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology
Animals
Humans
MESH: Protein Binding
MESH: Animals
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
Molecular Biology
Creatine Kinase
030304 developmental biology
0303 health sciences
Liposome
MESH: Creatine Kinase
MESH: Humans
MESH: Porins
Adenine nucleotide translocator
MESH: Chickens
Cell Biology
Surface Plasmon Resonance
Mitochondria
MESH: Surface Plasmon Resonance
Microscopy, Electron
MESH: Cattle
chemistry
Ionic strength
MESH: Calcium
Porin
biology.protein
Recombinant DNA
Cattle
Bacterial outer membrane
Chickens
030217 neurology & neurosurgery
Protein Binding
Subjects
Details
- Language :
- English
- ISSN :
- 00219258 and 1083351X
- Database :
- OpenAIRE
- Journal :
- Journal of Biological Chemistry, Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2001, 276 (51), pp.48027-30. ⟨10.1074/jbc.M106524200⟩
- Accession number :
- edsair.doi.dedup.....eac368c285d4ba73c4205c15115d53c9