Back to Search
Start Over
Evaluation of [C-11]-DAA1106 for imaging and quantification of neuro inflammation in a rat model of herpes encephalitis
- Source :
- Nuclear Medicine and Biology, 37(1), 9-15. ELSEVIER SCIENCE INC
- Publication Year :
- 2010
- Publisher :
- ELSEVIER SCIENCE INC, 2010.
-
Abstract
- Many neurological and psychiatric disorders are associated with neuroinflammation. Positron emission tomography (PET) with [(11)C]-PK11195 can be used to study neuroinflammation in these disorders. However, [(11)C]-PK11195 may not be sensitive enough to visualize mild neuroinflammation. As a potentially more sensitive PET tracer for neuroinflammation, [(11)C]-N-(2,5-dimethoxybenzyl)-N-(4-fluoro-2-phenoxyphenyl)-acetamide (DAA1106) was evaluated in a rat model of herpes encephalitis.Male Wistar rats were intranasally inoculated with HSV-1 (HSE) or phosphate-buffered saline (control). At Day 6 or Day 7 after inoculation, small-animal [(11)C]-DAA1106 PET scans were acquired, followed by ex vivo biodistribution. Arterial blood sampling was performed for quantification of uptake.In HSE rats, a significantly higher ex vivo, but not in vivo, uptake of [(11)C]-DAA1106 was found in almost all examined brain areas (24-71%, P.05), when compared to control rats. Pretreatment with unlabeled PK11195 effectively reduced [(11)C]-DAA1106 uptake in HSE rats (54-84%; P.001). The plasma and brain time-activity curves showed rapid uptake of [(11)C]-DAA1106 into tissue. The data showed a good fit to the Logan analysis but could not be fitted to a two-tissue compartment model.[(11)C]-DAA1106 showed a high and specific ex vivo uptake in the encephalitic rat brain. However, neuroinflammation could not be demonstrated in vivo by [(11)C]-DAA1106 PET. Quantification of the uptake of [(11)C]-DAA1106 using plasma sampling is not optimal, due to rapid tissue uptake, slow tissue clearance and low plasma activity.
- Subjects :
- Male
Cancer Research
Biodistribution
Pathology
medicine.medical_specialty
Positron emission tomography
Herpesvirus 1, Human
[C-11]-DAA1106
Central nervous system disease
HIGH-AFFINITY
POSITRON-EMISSION-TOMOGRAPHY
Neuroinflammation
In vivo
Acetamides
BINDING
medicine
Animals
Radiology, Nuclear Medicine and imaging
Tissue Distribution
PERIPHERAL BENZODIAZEPINE-RECEPTOR
Rats, Wistar
BRAIN
Inflammation
medicine.diagnostic_test
Microglia
business.industry
Phenyl Ethers
Herpes viruses
NEUROLOGICAL DISORDERS
medicine.disease
[C-11]-PK11195
DAA1106
Rats
Disease Models, Animal
medicine.anatomical_structure
PET
Positron-Emission Tomography
Molecular Medicine
Encephalitis, Herpes Simplex
LIGAND
business
Encephalitis
Ex vivo
TSPO
Subjects
Details
- Language :
- English
- ISSN :
- 09698051
- Volume :
- 37
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Nuclear Medicine and Biology
- Accession number :
- edsair.doi.dedup.....eabccb79875d7e44ebff3dddced4d524