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Expression of different CD8 isoforms on distinct human lymphocyte subpopulations
- Source :
- European Journal of Immunology. 21:1793-1800
- Publication Year :
- 1991
- Publisher :
- Wiley, 1991.
-
Abstract
- Human CD8+ lymphocyte subpopulations were analyzed for their expression of CD8 alpha and CD8 beta subunits. Investigations with uncloned peripheral blood lymphocytes as well as cloned human natural killer and T cell subpopulations demonstrate that CD3- natural killer cells, T cell receptor gamma/delta, and CD4+CD8+ T cell clones express exclusively CD8 alpha gene products. Structural analysis of CD8 molecules demonstrates that CD8 alpha+/beta- T lymphocytes surface express 75-kDa CD8 alpha/alpha homodimers whereas CD8 alpha/beta lymphocytes express concomittantly two CD8 isoforms of different molecular masses (67 kDa and 75 kDa, respectively). Peptide mapping of these latter two isoforms suggests that CD8 is expressed as alpha/alpha homodimers and alpha/beta heterodimers on CD8 alpha/beta+ cells. Importantly, we found that the two CD8 isoforms behave functionally different. Thus, in contrast to CD8 alpha/beta+/CD8 alpha/alpha+ T lymphocytes, cytolytic activity of CD8 alpha/beta-/CD8 alpha/alpha+ T cell clones was not inhibited by anti-CD8 monoclonal antibodies and the latter were not induced to proliferate following CD3/CD8 cross-linking.
- Subjects :
- Antigens, Differentiation, T-Lymphocyte
CD3 Complex
CD8 Antigens
T cell
Lymphocyte
Immunology
T-cell receptor
Receptors, Antigen, T-Cell
Antibodies, Monoclonal
Alpha (ethology)
T lymphocyte
Biology
Peptide Mapping
Precipitin Tests
Molecular biology
Natural killer cell
medicine.anatomical_structure
Antigens, CD
T-Lymphocyte Subsets
medicine
Humans
Immunology and Allergy
Beta (finance)
CD8
Subjects
Details
- ISSN :
- 15214141 and 00142980
- Volume :
- 21
- Database :
- OpenAIRE
- Journal :
- European Journal of Immunology
- Accession number :
- edsair.doi.dedup.....eab51e951bc7aed335425d643ce245ea